|
|
1.17.1.1 |
Description |
CDP-4-dehydro-6-deoxyglucose reductase |
Alternative names |
CDP-4-keto-6-deoxy-D-glucose-3-dehydrase;
CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase;
CDP-4-keto-6-deoxy-D-glucose-3-dehydrogenase system. |
Catalyzed reaction |
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ |
Cofactor |
Pyridoxamine-phosphate and iron-sulfur cluster. |
Comments |
The enzyme consists of two proteins. One forms an enzyme-bound adduct of the CDP-4-dehydro-6-deoxyglucose with pyridoxamine phosphate, in which the 3-hydroxy group has been removed. The second catalyses the reduction of this adduct by NAD(P)H and release of the CDP-4-dehydro-3,6-dideoxy-D-glucose and pyridoxamine phosphate. |
|
The first protein, termed E1, is CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase, which is a pyridoxamine 5'-phosphate dependent iron-sulfur protein. The second protein, CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase, formally called CDP-6-deoxy-delta(3,4)-glucoseen reductase (E3), is an NADH dependent [2Fe-2S]-containing flavoenzyme. |
PDB |
3BCX; |
Organisms |
-Eubacteria |
| |
Family |
|
| |
Links |
Enzyme (activities) 1.17.1.1
BRENDA (activities) 1.17.1.1
KEGG (pathways) 1.17.1.1
PLPMDB (PLP mutants) 1.17.1.1
|
| |
References |
Agnihotri G, Liu YN, Paschal BM, Liu HW. (2004) Identification of an unusual [2Fe-2S]-binding motif in the CDP-6-deoxy-D-glycero-l-threo-4-hexulose-3-dehydrase from Yersinia pseudotuberculosis: implication for C-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexoses Biochemistry 43 14265-74. Chen, X.M.; Ploux, O.; Liu, H.W. (1996) Biosynthesis of 3,6-dideoxyhexoses: in vivo and in vitro evidence for protein-protein interaction between CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase (E1) and its reductase (E3) Biochemistry 35 16412-20. Lei Y.; Ploux, O.; Liu, H.W. (1995) Mechanistic studies on CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase identification of His-220 as the active-site base by chemical modification and site-directed mutagenesis Biochemistry 34 4643-54. Gonzalez-Porque, P.; Strominger, J.L. (1972) Introducting of the 3-deoxy group in 3,6-dideoxyhexoses: a novel cofactor function for pyridoxamine-5'-phosphate Proc Natl Acad Sci U S A 69 1625-8. Articles on 1.17.1.1 |
| |
last changed |
2017/10/10 14:11 |
|