B6db activities : 1_17_1

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	1.17.1.1
Description	CDP-4-dehydro-6-deoxyglucose reductase.
Alternative names	CDP-4-keto-6-deoxy-D-glucose-3-dehydrase CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase CDP-4-keto-6-deoxy-D-glucose-3-dehydrogenase system
Catalyzed reaction	CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+
Cofactor	Pyridoxamine-phosphate and iron-sulfur cluster.
Comments	The enzyme consists of two proteins. One forms an enzyme-bound adduct of the CDP-4-dehydro-6-deoxyglucose with pyridoxamine phosphate, in which the 3-hydroxy group has been removed. The second catalyses the reduction of this adduct by NAD(P)H and release of the CDP-4-dehydro-3,6-dideoxy-D-glucose and pyridoxamine phosphate.
	The first protein, termed E1, is CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase, which is a pyridoxamine 5'-phosphate dependent iron-sulfur protein. The second protein, CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase, formally called CDP-6-deoxy-delta(3,4)-glucoseen reductase (E3), is an NADH dependent [2Fe-2S]-containing flavoenzyme.
PDB	3BCX;
Organisms	-Bacteria
Family	1.17.1.1 (6)

Links	Enzyme (activities) 1.17.1.1 BRENDA (activities) 1.17.1.1 KEGG (pathways) 1.17.1.1 PLPMDB (PLP mutants) 1.17.1.1

References	Chen, X.M.; Ploux, O.; Liu, H.W. (1996) Biosynthesis of 3,6-dideoxyhexoses: in vivo and in vitro evidence for protein-protein interaction between CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase (E1) and its reductase (E3) Biochemistry 35 16412-20.. Lei Y.; Ploux, O.; Liu, H.W. (1995) Mechanistic studies on CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-dehydrase identification of His-220 as the active-site base by chemical modification and site-directed mutagenesis Biochemistry 34 4643-54.. Gonzalez-Porque, P.; Strominger, J.L. (1972) Introducting of the 3-deoxy group in 3,6-dideoxyhexoses: a novel cofactor function for pyridoxamine-5'-phosphate Proc Natl Acad Sci U S A 69 1625-8.. Articles on 1.17.1.1

last changed	2008/05/29 09:27

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