Activities | Families | Sequences | Fold types | References | Help
B6db activities:
Description Glycine hydroxymethyltransferase.
Alternative names Serine hydroxymethyltransferase;
Serine aldolase;
Threonine aldolase;
Serine hydroxymethylase.
Catalyzed reaction 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.
Cofactor Pyridoxal-phosphate.
Comments -!- Also catalyzes the reaction of glycine with acetaldehyde to form L-threonine, and with 4-trimethylammoniobutanal to form 3-hydroxy- N6,N6,N6-trimethyl-L-lysine.
Prosite PROSITE; PDOC00090;
PDB 1BJ4; 1DFO; 1EJI; 1EQB; 1KKJ; 1KKP; 1KL2;
Organisms -Eubacteria -Archea -Plants -Fungi -Metazoa -Human

Family (70)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Jiang W, Xia B, Liu Z. (2013) A serine hydroxymethyltransferase from marine bacterium Shewanella algae: Isolation, purification, characterization and l-serine production. Microbiol Res 168 477-84.

 Wei Jiang, Bingzhao Xia, Junjie Huang and Ziduo Liu (2013) Characterization of a serine hydroxymethyltransferase for l-serine enzymatic production from Pseudomonas plecoglossicida World Journal of Microbiology and Biotechnology 29 2067-2076.

 Liu S, Kandoth PK, Warren SD, Yeckel G, Heinz R, Alden J, Yang C, Jamai A, El-Mellouki T, Juvale PS, Hill J, Baum TJ, Cianzio S, Whitham SA, Korkin D, Mitchum MG, Meksem K. (2012) A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens. Nature 492 256-60.

 Waditee-Sirisattha R1, Sittipol D, Tanaka Y, Takabe T. (2012) Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance. FEMS Microbiol Lett. 333 46-53.

 Shen P, Chao H, Jiang C, Long Z, Wang C, Wu B. (2010) Enhancing production of L-serine by increasing the glyA gene expression in Methylobacterium sp. MB200. Appl Biochem Biotechnol. 160 740-50.

 Sopitthummakhun K, Maenpuen S, Yuthavong Y, Leartsakulpanich U, Chaiyen P. (2009) Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues. FEBS J. 276 4023-36.

 Vatsyayan, R.; Roy, U. (2007) Molecular cloning and biochemical characterization of Leishmania donovani serine hydroxymethyltransferase Protein Expr Purif. 52 433-40.

 Mukherjee M, Sievers SA, Brown MT, Johnson PJ. (2006) Identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of Trichomonas vaginalis Eukaryot Cell 5 2072-8.

 Schirch V, Szebenyi DM. (2005) Serine hydroxymethyltransferase revisited. Curr Opin Chem Biol. 2005 Oct;9(5): 9 482-7.

 Angelaccio S, Chiaraluce R, Consalvi V, Buchenau B, Giangiacomo L, Bossa F, Contestabile R. (2003) Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii J Biol Chem 278 41789-97.

 Chaturvedi S, Bhakuni V. (2003) Unusual structural, functional, and stability properties of serine hydroxymethyltransferase from Mycobacterium tuberculosis J Biol Chem 278 40793-805.

 Trivedi, V.; Gupta, A.; Jala, V. R.; Saravanan, P.; Rao, G. S.; Rao, N. A.; Savithri, H. S.; Subramanya, H. S. (2002) Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism J Biol Chem 277 17161-9.

 Capelluto, D. G.; Hellman, U.; Cazzulo, J. J.; Cannata, J. J. (2000) Purification and some properties of serine hydroxymethyltransferase from Trypanosoma cruzi Eur J Biochem 267 712-9.

 Scarsdale, J. N.; Radaev, S.; Kazanina, G.; Schirch, V.; Wright, H. T. (2000) Crystal structure at 2.4 A resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate J Mol Biol 296 155-68.

 Szebenyi, D. M.; Liu, X.; Kriksunov, I. A.; Stover, P. J.; Thiel, D. J. (2000) Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers Biochemistry 39 13313-23.

 Scarsdale, J. N.; Kazanina, G.; Radaev, S.; Schirch, V.; Wright, H. T. (1999) Crystal structure of rabbit cytosolic serine hydroxymethyltransferase at 2.8 A resolution: mechanistic implications Biochemistry 38 8347-58.

 Delle Fratte, S.; White, R. H.; Maras, B.; Bossa, F.; Schirch, V. (1997) Purification and properties of serine hydroxymethyltransferase from Sulfolobus solfataricus J Bacteriol 179 7456-61.

 Jagath-Reddy, J.; Ganesan, K.; Savithri, H. S.; Datta, A.; Rao, N. A. (1995) cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase Eur J Biochem 230 533-7.

 Kruschwitz, H.; Ren, S.; Di Salvo, M.; Schirch, V. (1995) Expression, purification, and characterization of human cytosolic serine hydroxymethyltransferase Protein Expr Purif 6 411-6.

 Schirch, L. (1982) Serine hydroxymethyltransferase Adv Enzymol Relat Areas Mol Biol 53 83-112.

Articles on
last changed 2010/01/27 18:04

B6db activities