B6db activities : 2_1_2

	Activities \| Families \| Sequences \| Fold types \| References \| Help


	2.1.2.1
Description	Glycine hydroxymethyltransferase.
Alternative names	Serine hydroxymethyltransferase. Serine aldolase. Threonine aldolase. Serine hydroxymethylase.
Catalyzed reaction	5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.
Cofactor	Pyridoxal-phosphate.
Comments	-!- Also catalyzes the reaction of glycine with acetaldehyde to form L-threonine, and with 4-trimethylammoniobutanal to form 3-hydroxy- N6,N6,N6-trimethyl-L-lysine.
Prosite	PROSITE; PDOC00090;
PDB	1BJ4; 1DFO; 1EJI; 1EQB; 1KKJ; 1KKP; 1KL2;
Organisms	-Bacteria -Archea -Plants -Fungi -Metazoa -Human
Family	2.1.2.1 (63)

Links	Enzyme (activities) 2.1.2.1 BRENDA (activities) 2.1.2.1 KEGG (pathways) 2.1.2.1 PLPMDB (PLP mutants) 2.1.2.1

References	Vatsyayan, R.; Roy, U. (2007) Molecular cloning and biochemical characterization of Leishmania donovani serine hydroxymethyltransferase Protein Expr Purif. 52 433-40. Mukherjee M, Sievers SA, Brown MT, Johnson PJ. (2006) Identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of Trichomonas vaginalis Eukaryot Cell 5 2072-8. Schirch V, Szebenyi DM. (2005) Serine hydroxymethyltransferase revisited. Curr Opin Chem Biol. 2005 Oct;9(5): 9 482-7. Angelaccio S, Chiaraluce R, Consalvi V, Buchenau B, Giangiacomo L, Bossa F, Contestabile R. (2003) Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii J Biol Chem 278 41789-97. Chaturvedi S, Bhakuni V. (2003) Unusual structural, functional, and stability properties of serine hydroxymethyltransferase from Mycobacterium tuberculosis J Biol Chem 278 40793-805. Trivedi, V.; Gupta, A.; Jala, V. R.; Saravanan, P.; Rao, G. S.; Rao, N. A.; Savithri, H. S.; Subramanya, H. S. (2002) Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism J Biol Chem 277 17161-9. Capelluto, D. G.; Hellman, U.; Cazzulo, J. J.; Cannata, J. J. (2000) Purification and some properties of serine hydroxymethyltransferase from Trypanosoma cruzi Eur J Biochem 267 712-9. Scarsdale, J. N.; Radaev, S.; Kazanina, G.; Schirch, V.; Wright, H. T. (2000) Crystal structure at 2.4 A resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate J Mol Biol 296 155-68. Szebenyi, D. M.; Liu, X.; Kriksunov, I. A.; Stover, P. J.; Thiel, D. J. (2000) Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers Biochemistry 39 13313-23. Scarsdale, J. N.; Kazanina, G.; Radaev, S.; Schirch, V.; Wright, H. T. (1999) Crystal structure of rabbit cytosolic serine hydroxymethyltransferase at 2.8 A resolution: mechanistic implications Biochemistry 38 8347-58. Delle Fratte, S.; White, R. H.; Maras, B.; Bossa, F.; Schirch, V. (1997) Purification and properties of serine hydroxymethyltransferase from Sulfolobus solfataricus J Bacteriol 179 7456-61. Jagath-Reddy, J.; Ganesan, K.; Savithri, H. S.; Datta, A.; Rao, N. A. (1995) cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase Eur J Biochem 230 533-7. Kruschwitz, H.; Ren, S.; Di Salvo, M.; Schirch, V. (1995) Expression, purification, and characterization of human cytosolic serine hydroxymethyltransferase Protein Expr Purif 6 411-6. Schirch, L. (1982) Serine hydroxymethyltransferase Adv Enzymol Relat Areas Mol Biol 53 83-112. Articles on 2.1.2.1

last changed	2007/09/20 18:43

Family