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B6db activities:
Description O-acetylhomoserine (thiol)-lyase.
Alternative names O-acetylhomoserine aminocarboxypropyltransferase;
Methionine synthase;
O-acetylhomoserine sulfhydrylase;
OAH sulfhydrylase.
Catalyzed reaction O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.
Cofactor Pyridoxal-phosphate
Comments -!- Also reacts with other thiols or H(2)S, producing homocysteine or thioethers.
-!- The name methionine synthase is more commonly applied to EC
-!- An enzyme from baker's yeast also catalyzes the reaction of EC, more slowly.
Prosite PROSITE; PDOC00677;
Organisms -Eubacteria -Archea -Fungi

Family (23)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Shimizu H, Yamagata S, Masui R, Inoue Y, Shibata T, Yokoyama S, Kuramitsu S, Iwama T. (2001) Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product Biochim Biophys Acta 1549 1-72.

 Yamagata, S.; Ichioka, K.; Goto, K.; Mizuno, Y; Iwama, T. (2001) Occurrence of transsulfuration in synthesis of L-homocysteine in an extremely thermophilic bacterium, Thermus thermophilus HB8 J Bacteriol 183 2086-92.

 Bourhy, P.; Martel, A.; Margarita, D.; Saint Girons, I.; Belfaiza, J. (1997) Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited J Bacteriol 179 4396-4398.

 Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R. (1994) Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine.O-acetylhomoserine sulfhydrylase of the yeast Appl Microbiol Biotechnol 42 92-99.

 Brzywczy, J.; Yamagata, S.; Paszewski, A. (1993) Comparative studies on O-acetylhomoserine sulfhydrylase: physiological role and characterization of the Aspergillus nidulans enzyme Acta Biochim Pol 40 421-8.

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last changed 2010/01/28 11:07

B6db activities