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B6db activities: 2.6.1.1

2.6.1.1
Description Aspartate aminotransferase
Alternative names Transaminase A;
Glutamic--oxaloacetic transaminase;
Glutamic--aspartic transaminase;
AspAT;
GOT.
Catalyzed reaction L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor Pyridoxal-phosphate.
Comments -!- Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. This activity can be formed from EC 2.6.1.57 by controlled proteolysis.
Subfamily a includes eukariotic enzymes (both cytosolic and organellar).

Subfamily b encompasses proteins from gram-positive bacteria and from archaea.

Subfamily c is a compact subgroup including the E. coli AAT and other enzymes from proteobacteria.

Subfamily d represents a very distinct subgroup of bacterial enzymes based on the functionally validated sequence from Corynebacterium glutamicum.

Subfamily e is yet another very distinct group, including enzymes from thermophilic Archaea and from Eubacteria.

Subfamily f (distantly related to subfamily b) is based on a very divergent sequence from Bacillus.

Prosite PROSITE; PDOC00098;
PDB 1AAT; 1AAW; 1ASL; 1ARG; 1ARS; 1CQ6; 7AAT; 1J32; 1YAA; 3MEB; 5IWQ; 5C6U; 3HLM; 2X5F;
Organisms -Eubacteria -Archea -Plants -Fungi -Metazoa -Human
 

Family 

2.6.1.1_a (40) 2.6.1.1_b (30) 2.6.1.1_c (11) 2.6.1.1_d (10) 2.6.1.1_e (13) 2.6.1.1_f (12)
 
Links Enzyme (activities) 2.6.1.1
BRENDA (activities) 2.6.1.1
KEGG (pathways) 2.6.1.1
PLPMDB (PLP mutants) 2.6.1.1
 
References
 Jansen RS, Mandyoli L, Hughes R, Wakabayashi S, Pinkham JT, Selbach B, Guinn KM, Rubin EJ, Sacchettini JC, Rhee KY (2020) Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis Nat Commun 11 1960.

 Sheng Hu S, Zhang X, Lu Y, Lin Y-C, Xie D-F, Fang H, Huang J, Mei L-H (2017) Cloning, expression and characterization of an aspartate aminotransferase gene from Lactobacillus brevis CGMCC 1306 Biotechnology & Biotechnological Equipment 31 1219-30.

 Son HF, Kim KJ (2016) Structural Insights into a Novel Class of Aspartate Aminotransferase from Corynebacterium glutamicum PLoS One 11 e0158402.

 Abendroth J, Choi R, Wall A, Clifton MC, Lukacs CM, Staker BL, Van Voorhis W, Myler P, Lorimer DD, Edwards TE (2015) Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia Acta Crystallogr F Struct Biol Commun 71 566-71.

 Xu X, Gu L, He P, Zhou R (2015) Characterization of five putative aspartate aminotransferase genes in the N2-fixing heterocystous cyanobacterium Anabaena sp. strain PCC 7120 Microbiology 161 1219-30.

 Nadeem, M.S.; Rashid, N; Iqbal, M.; Gardner, Q.A.; Akhtar, M. (2011) First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis) Biologia 66 1202-1210.

 Wu HJ, Yang Y, Wang S, Qiao JQ, Xia YF, Wang Y, Wang WD, Gao SF, Liu J, Xue PQ, Gao XW. (2011) Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 FEBS J. 278 1345-57.

 Han Q, Cai T, Tagle DA, Li J. (2010) Structure, expression, and function of kynurenine aminotransferases in human and rodent brains Cell Mol Life Sci. 67 353-68.

 Kameya M, Arai H, Ishii M, Igarashi Y. (2010) Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6--novel types of alanine or glycine aminotransferase: enzymes and catalysis FEBS J 277 1876-85.

 Donini S, Ferrari M, Fedeli C, Faini M, Lamberto I, Marletta AS, Mellini L, Panini M, Percudani R, Pollegioni L, Caldinelli L, Petrucco S, Peracchi A. (2009) Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism Biochem J 422 265-72.

 Lee DK, Hwang JY, Cho HY, Kong KH (2009) A Thermostable Aspartate Aminotransferase from Aeropyrum pernix K1 Bull. Korean Chem. Soc. 30 31433146.

 Koma D, Sawai T, Hara R, Harayama S, Kino K. (2008) Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives Appl Microbiol Biotechnol 79 775-84.

 Koma D, Sawai T, Hara R, Harayama S, Kino K. (2008) Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives Appl Microbiol Biotechnol 79 775-84.

 Seetharamappa J, Oke M, Liu H, McMahon SA, Johnson KA, Carter L, Dorward M, Zawadzki M, Overton IM, van Niekirk CA, Graham S, Botting CH, Taylor GL, White MF, Barton GJ, Coote PJ, Naismith JH (2007) Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase Acta Crystallogr Sect F Struct Biol Cryst Commun 63 452-6.

 Marienhagen J, Kennerknecht N, Sahm H, Eggeling L. (2005) Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum J Bacteriol 187 7639-46.

 Vernal, J.; Cazzulo, J.; Nowicki, C. (2003) Cloning and heterologous expression of a broad specificity aminotransferase of Leishmania mexicana promastigotes1 FEMS Microbiol Lett 229 217-22.

 Ward, D.E.; de Vos, W.M.; van der Oost, J. (2002) Molecular analysis of the role of two aromatic aminotransferases and a broad-specificity aspartate aminotransferase in the aromatic amino acid metabolism of Pyrococcus furiosus Archaea 1 133-41.

 Birolo, L.; Tutino, M. L.; Fontanella, B.; Gerday, C.; Mainolfi, K.; Pascarella, S.; Sannia, G.; Vinci, F.; Marino, G. (2000) Aspartate aminotransferase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125. Cloning, expression, properties, and molecular modelling Eur J Biochem 267 2790-802.

 Gebhardt, J.S.; Wadsworth, G.J.; Matthews, B.F. (1998) Characterization of a single soybean cDNA encoding cytosolic and glyoxysomal isozymes of aspartate aminotransferase Plant Mol Biol 37 99-108.

 Jeffery, C. J.; Barry, T.; Doonan, S.; Petsko, G. A.; Ringe, D. (1998) Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase Protein Sci 7 1380-7.

 O'Farrell, P. A.; Sannia, G.; Walker, J. M.; Doonan, S. (1997) Cloning and sequencing of aspartate aminotransferase from Thermus aquaticus YT1 Biochem Biophys Res Commun 239 810-5.

 Bartsch, K.; Schneider, R.; Schulz, A. (1996) Stereospecific production of the herbicide phosphinothricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process Appl Environ Microbiol 62 3794-9.

 Okamoto, A.; Kato, R.; Masui, R.; Yamagishi, A.; Oshima, T.; Kuramitsu, S. (1996) An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 J Biochem (Tokyo) 119 135-44.

 Tanaka, T.; Yamamoto, S.; Moriya, T.; Taniguchi, M.; Hayashi, H.; Kagamiyama, H.; Oi, S. (1994) Aspartate aminotransferase from a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase family J Biochem (Tokyo) 115 309-17.

 Reynolds, P. H.; Smith, L. A.; Dickson, J. M.; Jones, W. T.; Jones, S. D.; Rodber, K. A.; Carne, A.; Liddane, C. P. (1992) Molecular cloning of a cDNA encoding aspartate aminotransferase-P2 from lupin root nodules Plant Mol Biol 19 465-72.

 Udvardi, M. K.; Kahn, M. L. (1991) Isolation and analysis of a cDNA clone that encodes an alfalfa (Medicago sativa) aspartate aminotransferase Mol Gen Genet 231 97-105.

 Mattes, U.; Jaussi, R.; Ziak, M.; Juretic, N.; Lindenmann, J. M.; Christen, P. (1989) Structure of cDNA of cytosolic aspartate aminotransferase of chicken and its expression in E. coli Biochimie 71 411-6.

 Nagashima, F.; Tanase, S.; Fukumoto, Y.; Joh, T.; Nomiyama, H.; Tsuzuki, T.; Shimada, K.; Kuramitsu, S.; Kagamiyama, H.; Morino, Y. (1989) cDNA cloning and expression of pig cytosolic aspartate aminotransferase in Escherichia coli: amino-terminal heterogeneity of expressed products and lack of its correlation with enzyme function Biochemistry 28 1153-60.

 Horio, Y.; Tanaka, T.; Taketoshi, M.; Nagashima, F.; Tanase, S.; Morino, Y.; Wada, H. (1988) Rat cytosolic aspartate aminotransferase: molecular cloning of cDNA and expression in Escherichia coli J Biochem (Tokyo) 103 797-804.

 Marino, G.; Nitti, G.; Arnone, M. I.; Sannia, G.; Gambacorta, A.; De Rosa, M. (1988) Purification and characterization of aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus J Biol Chem 263 12305-9.

 Martini, F.; Angelaccio, S.; Barra, D.; Pascarella, S.; Maras, B.; Doonan, S.; Bossa, F. (1985) The primary structure of mitochondrial aspartate aminotransferase from human heart Biochim Biophys Acta 832 46-51.

 Kondo K, Wakabayashi S, Yagi T, Kagamiyama H. (1984) The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes Biochem Biophys Res Commun 122 62-7.

 Barra, D.; Bossa, F.; Doonan, S.; Fahmy, H. M.; Hughes, G. J.; Martini, F.; Petruzzelli, R.; Wittmann-Liebold, B. (1980) The cytosolic and mitochondrial aspartate aminotransferases from pig heart. A comparison of their primary structures, predicted secondary structures and some physical properties Eur J Biochem 108 405-14.

Articles on 2.6.1.1
 
last changed 2020/03/01 19:52

B6db activities