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B6db activities: 2.6.1.1

2.6.1.1
Description Aspartate aminotransferase.
Alternative names Transaminase A;
Glutamic--oxaloacetic transaminase;
Glutamic--aspartic transaminase;
AspAT;
GOT.
Catalyzed reaction L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor Pyridoxal-phosphate.
Comments -!- Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. This activity can be formed from EC 2.6.1.57 by controlled proteolysis.
Subfamily a includes eukariotic enzymes (both cytosolic and organellar).

Subfamily b encompasses proteins from gram-positive bacteria and from archaea.

Subfamily c is a compact subgroup including the E. coli AAT and other enzymes from proteobacteria.

Prosite PROSITE; PDOC00098;
PDB 1AAT; 1AAW; 1ASL; 1ARG; 1ARS; 1CQ6; 7AAT; 1J32;
Organisms -Eubacteria -Archea -Plants -Fungi -Metazoa -Human
 

Family 

2.6.1.1_a (39) 2.6.1.1_b (28) 2.6.1.1_c (11)
 
Links Enzyme (activities) 2.6.1.1
BRENDA (activities) 2.6.1.1
KEGG (pathways) 2.6.1.1
PLPMDB (PLP mutants) 2.6.1.1
 
References
 Muhammad Shahid Nadeem, Naeem Rashid, Muzaffar Iqbal, Qurra-tul-Ann Afza Gardner, Muhammad Akhtar (2011) First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis) Biologia 66 1202-1210.

 Wu HJ, Yang Y, Wang S, Qiao JQ, Xia YF, Wang Y, Wang WD, Gao SF, Liu J, Xue PQ, Gao XW. (2011) Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 FEBS J. 278 1345-57.

 Han Q, Cai T, Tagle DA, Li J. (2010) Structure, expression, and function of kynurenine aminotransferases in human and rodent brains Cell Mol Life Sci. 67 353-68.

 Kameya M, Arai H, Ishii M, Igarashi Y. (2010) Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6--novel types of alanine or glycine aminotransferase: enzymes and catalysis FEBS J 277 1876-85.

 Ward, D.E.; de Vos, W.M.; van der Oost, J. (2002) Molecular analysis of the role of two aromatic aminotransferases and a broad-specificity aspartate aminotransferase in the aromatic amino acid metabolism of Pyrococcus furiosus Archaea 1 133-41.

 Birolo, L.; Tutino, M. L.; Fontanella, B.; Gerday, C.; Mainolfi, K.; Pascarella, S.; Sannia, G.; Vinci, F.; Marino, G. (2000) Aspartate aminotransferase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125. Cloning, expression, properties, and molecular modelling Eur J Biochem 267 2790-802.

 Gebhardt, J.S.; Wadsworth, G.J.; Matthews, B.F. (1998) Characterization of a single soybean cDNA encoding cytosolic and glyoxysomal isozymes of aspartate aminotransferase Plant Mol Biol 37 99-108.

 Jeffery, C. J.; Barry, T.; Doonan, S.; Petsko, G. A.; Ringe, D. (1998) Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase Protein Sci 7 1380-7.

 O'Farrell, P. A.; Sannia, G.; Walker, J. M.; Doonan, S. (1997) Cloning and sequencing of aspartate aminotransferase from Thermus aquaticus YT1 Biochem Biophys Res Commun 239 810-5.

 Bartsch, K.; Schneider, R.; Schulz, A. (1996) Stereospecific production of the herbicide phosphinothricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process Appl Environ Microbiol 62 3794-9.

 Bartsch, K.; Schneider, R.; Schulz, A. (1996) Stereospecific production of the herbicide phosphinothricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process Appl Environ Microbiol 62 3794-9.

 Okamoto, A.; Kato, R.; Masui, R.; Yamagishi, A.; Oshima, T.; Kuramitsu, S. (1996) An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 J Biochem (Tokyo) 119 135-44.

 Tanaka, T.; Yamamoto, S.; Moriya, T.; Taniguchi, M.; Hayashi, H.; Kagamiyama, H.; Oi, S. (1994) Aspartate aminotransferase from a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase family J Biochem (Tokyo) 115 309-17.

 Reynolds, P. H.; Smith, L. A.; Dickson, J. M.; Jones, W. T.; Jones, S. D.; Rodber, K. A.; Carne, A.; Liddane, C. P. (1992) Molecular cloning of a cDNA encoding aspartate aminotransferase-P2 from lupin root nodules Plant Mol Biol 19 465-72.

 Udvardi, M. K.; Kahn, M. L. (1991) Isolation and analysis of a cDNA clone that encodes an alfalfa (Medicago sativa) aspartate aminotransferase Mol Gen Genet 231 97-105.

 Mattes, U.; Jaussi, R.; Ziak, M.; Juretic, N.; Lindenmann, J. M.; Christen, P. (1989) Structure of cDNA of cytosolic aspartate aminotransferase of chicken and its expression in E. coli Biochimie 71 411-6.

 Nagashima, F.; Tanase, S.; Fukumoto, Y.; Joh, T.; Nomiyama, H.; Tsuzuki, T.; Shimada, K.; Kuramitsu, S.; Kagamiyama, H.; Morino, Y. (1989) cDNA cloning and expression of pig cytosolic aspartate aminotransferase in Escherichia coli: amino-terminal heterogeneity of expressed products and lack of its correlation with enzyme function Biochemistry 28 1153-60.

 Horio, Y.; Tanaka, T.; Taketoshi, M.; Nagashima, F.; Tanase, S.; Morino, Y.; Wada, H. (1988) Rat cytosolic aspartate aminotransferase: molecular cloning of cDNA and expression in Escherichia coli J Biochem (Tokyo) 103 797-804.

 Martini, F.; Angelaccio, S.; Barra, D.; Pascarella, S.; Maras, B.; Doonan, S.; Bossa, F. (1985) The primary structure of mitochondrial aspartate aminotransferase from human heart Biochim Biophys Acta 832 46-51.

 Kondo K, Wakabayashi S, Yagi T, Kagamiyama H. (1984) The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes Biochem Biophys Res Commun 122 62-7.

 Barra, D.; Bossa, F.; Doonan, S.; Fahmy, H. M.; Hughes, G. J.; Martini, F.; Petruzzelli, R.; Wittmann-Liebold, B. (1980) The cytosolic and mitochondrial aspartate aminotransferases from pig heart. A comparison of their primary structures, predicted secondary structures and some physical properties Eur J Biochem 108 405-14.

Articles on 2.6.1.1
 
last changed 2014/03/03 17:34

B6db activities