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B6db activities: 2.6.1.100

2.6.1.100
Description L-glutamine:2-deoxy-scyllo-inosose aminotransferase
Alternative names L-glutamine:2-deoxy-scyllo-inosose aminotransferase;
btrR;
neoB;
kanB.
Catalyzed reaction L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
Cofactor Pyridoxal-phosphate.
Comments Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.101, L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase.
PDB 2C7T; 2C81;
Organisms -Eubacteria
 

Family 

2.6.1.100 (7)
 
Links Enzyme (activities) 2.6.1.100
BRENDA (activities) 2.6.1.100
KEGG (pathways) 2.6.1.100
PLPMDB (PLP mutants) 2.6.1.100
 
References
 Cui L, Zhu Y, Guan X, Deng Z, Bai L, Feng Y (2016) De Novo Biosynthesis of β-Valienamine in Engineered Streptomyces hygroscopicus 5008 ACS Synth Biol 5 15-20.

 Jnawali HN, Subba B, Liou K, Sohng JK. (2009) Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr Biotechnol Lett 31 869-75.

 Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB. (2006) Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis Proteins 65 220-30.

 Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB. (2005) The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine Org Biomol Chem 3 1410-8.

 Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K. (2005) Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius Bioorg Med Chem Lett 15 89-92.

 Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. (2005) Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 J Antibiot (Tokyo) 58 766-74.

 Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K. (2002) Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans J Antibiot (Tokyo) 55 707-714.

Articles on 2.6.1.100
 
last changed 2017/11/03 17:50

B6db activities