|
|
2.6.1.102 |
Description |
GDP-perosamine synthase |
Alternative names |
perosamine synthetase;
GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase;
RfbE;
Per. |
Catalyzed reaction |
GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate <=> GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate. |
Cofactor |
Pyridoxal-phosphate. |
Comments |
D-perosamine is one of several dideoxy sugars found in the O-specific polysaccharide of the lipopolysaccharide component of the outer membrane of Gram-negative bacteria.
The enzyme catalyzes the final step in GDP-perosamine synthesis. |
|
In bacteria,D-perosamine is used for the glycosylation of products such as antibiotics and lipopolysaccharides.
Perosamine synthase occurs in a wide variety of microorganisms, including pathogens, suggesting that the enzyme performs a function crucial for the normal life cycle and/or virulence of many species. |
PDB |
3BN1; |
Organisms |
-Eubacteria |
| |
Family |
|
| |
Links |
Enzyme (activities) 2.6.1.102
BRENDA (activities) 2.6.1.102
KEGG (pathways) 2.6.1.102
PLPMDB (PLP mutants) 2.6.1.102
|
| |
References |
Cook PD, Holden HM. (2008) GDP-perosamine synthase: structural analysis and production of a novel trideoxysugar Biochemistry 47 2833-40. Zhao G, Liu J, Liu X, Chen M, Zhang H, Wang PG. (2007) Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro
Biochem Biophys Res Commun 363 525-30. Muller-Dieckmann, Hans-Joachim ; et al. (2003) Crystals and structures of perosamine synthase homologs United States Patent Application 20030101005 . Albermann, C.; Piepersberg, W.
(2001) Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine Glycobiology 11 655-61. Godfroid, F., Taminiau, B., Danese, I., Denoel, P., Tibor, A., Weynants, V., Cloeckaert, A., Godfroid, J.; Letesson J.J.
(1998) Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages Infect. Immun. 66 5485-5493. Articles on 2.6.1.102 |
| |
last changed |
2014/02/19 11:38 |
|