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Description (S)-3,5-dihydroxyphenylglycine transaminase
Alternative names (S)-3,5-dihydroxyphenylglycine transaminase.
L-p-hydroxyphenylglycine transaminase.
(S)-4-hydroxyphenylglycine amino transferase.
HpgT (gene name)
Catalyzed reaction (S)-3,5-dihydroxyphenylglycine + 2-oxoglutarate = 2-(3,5-dihydroxyphenyl)-2-oxoacetate + L-glutamate.

(S)-4-hydroxyphenylglycine + 4-hydroxyphenylpyruvate = 4-hydroxyphenyl-2-oxoacetate + L-tyrosine

Cofactor Pyridoxal-phosphate.
Comments The enzyme from the bacterium Amycolatopsis orientalis catalyses the first reaction in the reverse direction as part of the biosynthesis of the (S)-3,5-dihydroxyphenylglycine constituent of the glycopeptide antibiotic chloroeremomycin.
The enzyme from Amycolatopsis orientalis also transaminates L-4-hydroxyphenylglycine (second of the reactions above) and is involved in the synthesis of chloroeremomycin. A similar, characterized enzyme from Streptomyces lavendulae also transaminates L-4-hydroxyphenylglycine and acts in the pathway for the production of complestatin. These enzymes do not use D-p-hydroxyphenylglycine as a substrate and thus are totally distinct from EC
Organisms -Eubacteria

Family (0)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Tan B, Zhang Q, Zhu Y, Jin H, Zhang L, Chen S, Zhang C (2020) Deciphering Biosynthetic Enzymes Leading to 4-Chloro-6-Methyl-5,7-Dihydroxyphenylglycine, a Non-Proteinogenic Amino Acid in Totopotensamides ACS Chem Biol in press.

 Chiu, H. T.; Hubbard, B. K.; Shah, A. N.; Eide, J.; Fredenburg, R. A.; Walsh, C. T.; Khosla, C. (2001) Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster Proc Natl Acad Sci U S A 98 8548-53.

 Pfeifer V, Nicholson GJ, Ries J, Recktenwald J, Schefer AB, Shawky RM, Schröder J, Wohlleben W, Pelzer S (2001) A polyketide synthase in glycopeptide biosynthesis: the biosynthesis of the non-proteinogenic amino acid (S)-3,5-dihydroxyphenylglycine J Biol Chem 276 38370-7.

 Sandercock, A.M., Charles, E.H., Scaife, W., Kirkpatrick, P.N., O'Brien, S.W., Papageorgiou, E.A., Spencer, J.B., Williams, D.H. (2001) Biosynthesis of the di-meta-hydroxyphenylglycine constituent of the vancomycin-group antibiotic chloroeremomycin Chem Commun 2001 1252-1253.

 Hubbard BK, Thomas MG, Walsh CT. (2000) Biosynthesis of L-p-hydroxyphenylglycine, a non-proteinogenic amino acid constituent of peptide antibiotics Chem Biol 7 931-42.

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last changed 2019/05/17 10:02

B6db activities