Activities | Families | Sequences | Fold types | References | Help
B6db activities: 2.6.1.113

2.6.1.113
Description Putrescine--pyruvate transaminase
Alternative names Putrescine--pyruvate transaminase;
PATase;
SpuC (gene name)
Catalyzed reaction putrescine + pyruvate = 4-aminobutanal + L-alanine.
Cofactor Pyridoxal phosphate.
Comments The enzyme, studied in the bacterium Pseudomonas aeruginosa, participates in a putrescine degradation pathway.
Distinct from 2.6.1.82 because the preferred ketoacid substrate is pyruvate rather than 2-oxoglutarate.

As with 2.6.1.82, the 4-aminobutanal undergoes spontaneous cyclization, forming 1-pyrroline.

PDB 6HX9;
Organisms -Eubacteria
 

Family 

2.6.1.113 (0)
 
Links Enzyme (activities) 2.6.1.113
BRENDA (activities) 2.6.1.113
KEGG (pathways) 2.6.1.113
PLPMDB (PLP mutants) 2.6.1.113
 
References
 Galman JL, Gahloth D, Parmeggiani F, Slabu I, Leys D, Turner NJ (2018) Characterization of a Putrescine Transaminase From Pseudomonas putida and its Application to the Synthesis of Benzylamine Derivatives Front Bioeng Biotechnol 6 205.

 Galman,J.L., Slabu,I., Weise,N.J., Iglesias,C., Parmeggiani,F., Lloyd,R.C. and Turner,N.J. (2017) Biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases Green Chem 19 361-366.

 Wilding M, Walsh EF, Dorrian SJ, Scott C (2015) Identification of novel transaminases from a 12-aminododecanoic acid-metabolizing Pseudomonas strain Microb Biotechnol 8 665-72.

 Lu CD, Itoh Y, Nakada Y, Jiang Y. (2002) Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1 J Bacteriol 184 3765-73.

Articles on 2.6.1.113
 
last changed 2019/01/14 13:32

B6db activities