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B6db activities: 2.6.1.36

2.6.1.36
Description L-lysine aminotransferase.
Alternative names L-Lysine 6-aminotransferase;
Lysine-epsilon aminotransferase;
L-Lysine 6-transaminase.
Catalyzed reaction L-lysine + 2-oxoglutarate = 2-aminoadipate 6-semialdehyde + L-glutamate.
Cofactor Pyridoxal-phosphate.
Comments -!- The product (allysine) is converted into the intramolecularly dehydrated form, 1-piperideine 6-carboxylate.
The enzyme is involved in L-lysine catabolism. In actinomycetes, this enzyme is specific to the first step of the beta-lactam antibiotic biosynthetic pathway. An enzyme from Sphingomonas paucimobilis has been cloned (see Patel et al., 2000) but the sequence is not available from current primary databases.
PDB 2CJH; 2CJD; 2CIN;
Organisms -Eubacteria -Fungi
 

Family 

 
Links Enzyme (activities) 2.6.1.36
BRENDA (activities) 2.6.1.36
KEGG (pathways) 2.6.1.36
PLPMDB (PLP mutants) 2.6.1.36
 
References
 Mani Tripathi S, Ramachandran R. (2006) Overexpression, purification and crystallization of lysine epsilon-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv Acta Crystallogr Sect F Struct Biol Cryst Commun 62 572-5.

 Mani Tripathi S, Ramachandran R. (2006) Direct evidence for a glutamate switch necessary for substrate recognition: crystal structures of lysine epsilon-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv J Mol Biol 362 877-86.

 Fujii, T.; Narita, T.; Agematu, H.; Agata, N.; Isshiki, K. (2000) Characterization of L-lysine 6-aminotransferase and its structural gene from Flavobacterium lutescens IFO3084 J Biochem (Tokyo) 128 391-7.

 Patel, R.N. et al. (2000) Biocatalytic preparation of a chiral synthon for a vasopeptidase inhibitor: enzymatic conversion of N-2-[N-phenylmethoxy)carbonyl] L-homocysteinyl]-L-lysine (1-> 1 ')-disulfide to [4S-(4I,7I,10aJ)] 1-octahydro-5-oxo-4-[phenylmethoxy)carbonyl]amino]-7H-pyrido-[2,1-b] [1,3]thiazepine-7-carboxylic acid methyl ester by a novel L-lysine epsilon-aminotransferase Enzyme Microb Tech 27 376-389 .

 Coque, J. J.; Liras, P.; Laiz, L.; Martin, J. F. (1991) A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans J Bacteriol 173 6258-64.

 Tobin, M.B.; Kovacevic, S.; Madduri, K.; Hoskins, J.A.; Skatrud, P.L.; Vining, L.C.; Stuttard, C.; Miller, J.R. (1991) Localization of the lysine epsilon-aminotransferase (lat) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (pcbAB) genes from Streptomyces clavuligerus and production of lysine epsilon-aminotransferase activity in Escherichia coli J Bacteriol 173 6223-9.

Articles on 2.6.1.36
 
last changed 2010/03/11 16:16

B6db activities