B6db activities : 2_6_1

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	2.6.1.50
Description	Glutamine--scyllo-inosose aminotransferase.
Alternative names	L-glutamine:2-deoxy-scyllo-inosose aminotransferase; BtrR
Catalyzed reaction	L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone = 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol.
Cofactor	Pyridoxal-phosphate.
Comments	Enzymes with this activity are required for the biosynthesis of 2-deoxystreptamine antibiotics such as neomycin (in streptomycetes) or butyrosin (in Bacillus circulans).
PDB	2C7T; 2C81;
Organisms	-Bacteria
Family	2.6.1.50 (7)

Links	Enzyme (activities) 2.6.1.50 BRENDA (activities) 2.6.1.50 KEGG (pathways) 2.6.1.50 PLPMDB (PLP mutants) 2.6.1.50

References	Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB. (2006) Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis Proteins 65 220-30. Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB. (2005) The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine Org Biomol Chem 3 1410-8. Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K. (2005) Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius Bioorg Med Chem Lett 15 89-92. Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. (2005) Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 J Antibiot (Tokyo) 58 766-74. Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K. (2002) Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans J Antibiot (Tokyo) 55 707-714. Ahlert, J.; Distler, J.; Mansouri, K.; Piepersberg, W. (1997) Identification of stsC, the gene encoding the L-glutamine:scyllo- inosose aminotransferase from streptomycin-producing Streptomycetes Arch Microbiol 168 102-13.. Articles on 2.6.1.50

last changed	2007/11/30 14:05

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