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B6db activities: 2.6.1.50

2.6.1.50
Description Glutamine--scyllo-inosose aminotransferase.
Alternative names L-glutamine:2-deoxy-scyllo-inosose aminotransferase;
StsC;
BtrR.
Catalyzed reaction L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone = 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol.
Cofactor Pyridoxal-phosphate.
Comments Enzymes with this activity are required for the biosynthesis of 2-deoxystreptamine antibiotics such as neomycin (in streptomycetes) or butyrosin (in Bacillus circulans).
PDB 2C7T; 2C81;
Organisms -Eubacteria
 

Family 

2.6.1.50 (8)
 
Links Enzyme (activities) 2.6.1.50
BRENDA (activities) 2.6.1.50
KEGG (pathways) 2.6.1.50
PLPMDB (PLP mutants) 2.6.1.50
 
References
 Jnawali HN, Subba B, Liou K, Sohng JK. (2009) Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr Biotechnol Lett 31 869-75.

 Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB. (2006) Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis Proteins 65 220-30.

 Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB. (2005) The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine Org Biomol Chem 3 1410-8.

 Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K. (2005) Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius Bioorg Med Chem Lett 15 89-92.

 Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. (2005) Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 J Antibiot (Tokyo) 58 766-74.

 Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K. (2002) Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans J Antibiot (Tokyo) 55 707-714.

 Ahlert, J.; Distler, J.; Mansouri, K.; Piepersberg, W. (1997) Identification of stsC, the gene encoding the L-glutamine:scyllo- inosose aminotransferase from streptomycin-producing Streptomycetes Arch Microbiol 168 102-13.

Articles on 2.6.1.50
 
last changed 2010/03/11 16:23

B6db activities