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2.6.1.72 |
| Description |
D-4-hydroxyphenylglycine aminotransferase |
| Alternative names |
D-phenylglycine aminotransferase |
| Catalyzed reaction |
D-4-hydroxyphenylglycine + 2-oxoglutarate = 4-hydroxyphenylglyoxylate + L-glutamate. |
| Cofactor |
Pyridoxal-phosphate. |
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The enzyme from Pseudomonas strutzeri accepts as amino group donor only D-4-hydroxyphenylglycine or D-phenylglycine, whereas 2-oxoglutarate is the exclusive amino group acceptor and is converted into L-glutamate. |
| PDB |
2CY8; |
| Organisms |
-Eubacteria |
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| Links |
Enzyme (activities) 2.6.1.72
BRENDA (activities) 2.6.1.72
KEGG (pathways) 2.6.1.72
PLPMDB (PLP mutants) 2.6.1.72
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| References |
Walton CJ, Thiebaut F, Brunzelle JS, Couture JF, Chica RA
(2018) Structural determinants of the stereo-inverting activity of Pseudomonas stutzeri D-phenylglycine aminotransferase Biochemistry 57 5437-5446. Chantarasiri A, Meevootisom V, Isarangkul D, Wiyakrutta S.
(2012) Effective improvement of D-phenylglycine aminotransferase solubility by protein crystal contact engineering J Mol Microbiol Biotechnol 22 147-55. Müller U, van Assema F, Gunsior M, Orf S, Kremer S, Schipper D, Wagemans A, Townsend CA, Sonke T, Bovenberg R, Wubbolts M.
(2006) Metabolic engineering of the E. coli L-phenylalanine pathway for the production of D-phenylglycine (D-Phg) Metab Eng 8 196-208. Wiyakrutta S, Meevootisom V. (1997) A stereo-inverting D-phenylglycine aminotransferase from Pseudomonas stutzeri ST-201: purification, characterization and application for D-phenylglycine synthesis J Biotechnol 55 193-203. Van den Tweel, W.J.J.; Smits, J.P.; Ogg, R.L.H.P.; de Bont, J.A.M. (1988) The involvement of an enantioselctive transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine in Pseudomonas putida LW-4 Appl Microbiol Biotechnol 29 224-230. |
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| last changed |
2019/06/20 11:22 |
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