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B6db activities: 2.6.1.93

2.6.1.93
Description Neamine transaminase
Alternative names glutamate-6'-dehydroparomamine aminotransferase;
neamine:2-oxoglutarate aminotransferase;
btrB;
neoB;
kacL.
Catalyzed reaction neamine + 2-oxoglutarate = 6'-dehydroparomamine + L-glutamate
Cofactor Pyridoxal-phosphate.
Comments The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6'-hydroxy group of paromamine with an amino group.

The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2'-deamino-2'-hydroxyneamine transaminase, which leads to production of kanamycin A.

The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C.

The validated enzyme from Streptomyces kanamyceticus, which also possesses 2'-deamino-2'-hydroxyneamine transaminase activity and shows only a moderate identity to the enzyme from S. fradiae, is included in family 2.6.1.94.

PDB 6CBK; 6CBN;
Organisms -Eubacteria
 

Family 

2.6.1.93 (6)
 
Links Enzyme (activities) 2.6.1.93
BRENDA (activities) 2.6.1.93
KEGG (pathways) 2.6.1.93
PLPMDB (PLP mutants) 2.6.1.93
 
References
 Dow GT, Thoden JB, Holden HM (2018) The Three-Dimensional Structure of NeoB: An Aminotransferase Involved in the Biosynthesis of Neomycin Protein Sci 27 945-956.

 Clausnitzer D, Piepersberg W, Wehmeier UF. (2011) The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin J Appl Microbiol 111 642-51.

 Park, J.W., Park, S.R., Nepal, K.K., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., Kim, E.M., Kim, D., Sohng, J.K. and Yoon, Y.J. (2011) Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation. Nat Chem Biol. 7 843-52.

 Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB. (2007) Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. Chembiochem 8 283-8.

Articles on 2.6.1.93
 
last changed 2018/03/19 09:01

B6db activities