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2.6.1.96 |
Description |
4-aminobutyrate—pyruvate transaminase |
Alternative names |
GABA--pyruvate aminotransferase;
4-aminobutyrate--pyruvate aminotransferase;
GABA-T. |
Catalyzed reaction |
(1) 4-aminobutanoate + pyruvate = succinate semialdehyde + L-alanine
(2) 4-aminobutanoate + glyoxylate = succinate semialdehyde + glycine. |
Cofactor |
Pyridoxal phosphate. |
Comments |
The enzyme is found in plants that do not have the 2-oxoglutarate dependent enzyme (cf. EC 2.6.1.19).
The reaction with pyruvate is reversible while the reaction with glyoxylate only takes place in the forward direction. |
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The sequences are similar to 2.6.1.18 (beta-alanine--pyruvate aminotransferase) and indeed this enzyme, too, is an omega-amino acid--pyruvate aminotransferase. |
Organisms |
-Plants |
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Family |
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Links |
Enzyme (activities) 2.6.1.96
BRENDA (activities) 2.6.1.96
KEGG (pathways) 2.6.1.96
PLPMDB (PLP mutants) 2.6.1.96
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References |
Weber N, Gorwa-Grauslund M, Carlquist M. (2014) Exploiting cell metabolism for biocatalytic whole-cell transamination by recombinant Saccharomyces cerevisiae Appl Microbiol Biotechnol 98 4615-24. Trobacher CP, Clark SM, Bozzo GG, Mullen RT, DeEll JR, Shelp BJ (2012) Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases. Postharvest Biol Technol 75 106-113. Clark SM, Di Leo R, Dhanoa PK, Van Cauwenberghe OR, Mullen RT, Shelp BJ.
(2009) Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate J Exp Bot 60 1743-57. Palanivelu R, Brass L, Edlund AF, Preuss D. (2003) Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels Cell 114 47-59. Van Cauwenberghe, O.R.; Makhmoudova, A.; McLean, M.D.; Clark, S.M.; Shelp, B.J.
(2002) Plant pyruvate-dependent gamma-aminobutyrate transaminase:Identification of an Arabidopsis cDNA and its expression in Escherichia coli Can J Bot 80 933-41. Articles on 2.6.1.96 |
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last changed |
2014/02/14 11:33 |
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