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B6db activities: 2.6.1.96

2.6.1.96
Description 4-aminobutyrate—pyruvate transaminase
Alternative names GABA--pyruvate aminotransferase;
4-aminobutyrate--pyruvate aminotransferase;
GABA-T.
Catalyzed reaction (1) 4-aminobutanoate + pyruvate = succinate semialdehyde + L-alanine
(2) 4-aminobutanoate + glyoxylate = succinate semialdehyde + glycine.
Cofactor Pyridoxal phosphate.
Comments The enzyme is found in plants that do not have the 2-oxoglutarate dependent enzyme (cf. EC 2.6.1.19).

The reaction with pyruvate is reversible while the reaction with glyoxylate only takes place in the forward direction.

The sequences are similar to 2.6.1.18 (beta-alanine--pyruvate aminotransferase) and indeed this enzyme, too, is an omega-amino acid--pyruvate aminotransferase.
Organisms -Plants
 

Family 

2.6.1.96 (9)
 
Links Enzyme (activities) 2.6.1.96
BRENDA (activities) 2.6.1.96
KEGG (pathways) 2.6.1.96
PLPMDB (PLP mutants) 2.6.1.96
 
References
 Weber N, Gorwa-Grauslund M, Carlquist M. (2014) Exploiting cell metabolism for biocatalytic whole-cell transamination by recombinant Saccharomyces cerevisiae Appl Microbiol Biotechnol 98 4615-24.

 Trobacher CP, Clark SM, Bozzo GG, Mullen RT, DeEll JR, Shelp BJ (2012) Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases. Postharvest Biol Technol 75 106-113.

 Clark SM, Di Leo R, Dhanoa PK, Van Cauwenberghe OR, Mullen RT, Shelp BJ. (2009) Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate J Exp Bot 60 1743-57.

 Palanivelu R, Brass L, Edlund AF, Preuss D. (2003) Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels Cell 114 47-59.

 Van Cauwenberghe, O.R.; Makhmoudova, A.; McLean, M.D.; Clark, S.M.; Shelp, B.J. (2002) Plant pyruvate-dependent gamma-aminobutyrate transaminase:Identification of an Arabidopsis cDNA and its expression in Escherichia coli Can J Bot 80 933-41.

Articles on 2.6.1.96
 
last changed 2014/02/14 11:33

B6db activities