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Description Cysteine desulfurase
Alternative names IscS;
cysteine desulfurylase.
Catalyzed reaction L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Cofactor Pyridoxal-phosphate.
Comments The reaction shown is the first part of a catalytic process, which is completed by passing on the protein-bound sulfur to other acceptors.
In Azotobacter vinelandii, this sulfur provides the inorganic sulfide required for nitrogenous metallocluster formation. The enzyme is involved in the biosynthesis of iron-sulfur clusters, thio-nucleosides in tRNA, thiamine, biotin, lipoate and pyranopterin (molybdopterin) and functions by mobilizing sulfur.
Cysteine desulfurases are phylogenetically and functionally related to enzymes such as selenocystine lyases (C-DES type).
They are also phylogenetically related to selenocysteine lyases (EC, and often show some selenocysteine lyase activity.
Diseases Classical xanthinuria type II
PDB 1ECX; 1P3W; 6M4J;
Organisms -Eubacteria -Archea -Plants -Metazoa -Human

Family (41) (12) (10)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Liu L, Jiang S, Xing M, Chen C, Lai C, Li N, Liu , Wu D3, Gao H, Hong L, Tan P, Chen S, Deng Z, Wu G, Wang L (2020) Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System mBio 11 e00488-20.

 Hertweck C (2018) Enzymatic Thioamide Formation in Bacterial Antimetabolite Pathway Angew Chem Int Ed Engl 57 11574-11578.

 Wang Y, Liu Q, Zhou H, Chen X (2017) Expression, purification and function of cysteine desulfurase from Sulfobacillus acidophilus TPY isolated from deep-sea hydrothermal vent 3 Biotech 7 360.

 Ruiz M, Bettache A, Janicki A, Vinella D, Zhang CC, Latifi A. (2010) The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress FEBS J 277 3715-25.

 Marelja Z, Stöcklein W, Nimtz M, Leimkühler S. (2008) A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis J Biol Chem 283 25178-85.

 Neumann M, Stöcklein W, Walburger A, Magalon A, Leimkühler S (2007) Identification of a Rhodobacter capsulatus L-cysteine desulfurase that sulfurates the molybdenum cofactor when bound to XdhC and before its insertion into xanthine dehydrogenase Biochemistry 46 9586-95.

 You D, Wang L, Yao F, Zhou X, Deng Z. (2007) A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans Biochemistry 46 6126-33.

 Zeng J, Zhang Y, Liu Y, Zhang X, Xia L, Liu J, Qiu G (2007) Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans Biotechnol Lett 29 1983-90.

 Biederbick A, Stehling O, Rösser R, Niggemeyer B, Nakai Y, Elsässer HP, Lill R. (2006) Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation Mol Cell Biol 26 5675-87.

 Muhlenhoff, U.; Balk, J.; Richhardt, N.; Kaiser, J. T.; Sipos, K.; Kispal, G.; Lill, R. (2004) Functional characterisation of the eukaryotic cysteine desulfurase Nfs1p from S. cerevisiae J Biol Chem 279 36906-15.

 Mihara, H.; Esaki, N. (2002) Bacterial cysteine desulfurases: their function and mechanisms Appl Microbiol Biotechnol 60 12-23.

 Nilsson, K.; Lundgren, H.K.; Hagervall, T.G.; Bjork, G.R. (2002) The cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar typhimurium J Bacteriol 184 6830-5.

 Kaiser, J.T.; Clausen, T.; Bourenkow, G.P.; Bartunik, H.D.; Steinbacher, S.; Huber, R. (2000) Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly J Mol Biol 297 451-64.

 Kato, S.; Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N. (2000) Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation Biosci Biotechnol Biochem 64 2412-9.

 Kirby, J.; Wright, F.; Flint, H.J. (1998) A cysteine desulphurase gene from the cellulolytic rumen anaerobe Ruminococcus flavefaciens Biochim Biophys Acta 1386 233-7.

 Flint, D. H. (1996) Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase J Biol Chem 271 16068-74.

 Zheng, L.; White, R.H.; Cash, V.L.; Jack, R.F.; Dean, D.R. (1993) Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis Proc Natl Acad Sci U S A 90 2754-8.

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last changed 2020/05/08 01:58

B6db activities