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2.9.1.1 |
| Description |
L-seryl-tRNA(Sec) selenium transferase |
| Alternative names |
Selenocysteine synthase.
L-selenocysteinyl-tRNA(Sel) synthase;
L-selenocysteinyl-tRNA(Sec) synthase;
Cysteinyl-tRNA(Sel)-selenium transferase;
Cysteinyl-tRNA(Sec)-selenium transferase. |
| Catalyzed reaction |
L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + H(2)O + phosphate. |
| Cofactor |
Pyridoxal-phosphate. |
| Comments |
-!- Recognizes specifically tRNA(Sec)-species. Binding of tRNA(Sec) also occurs in the absence of the seryl group.
-!- 2-Aminoacryloyl-tRNA, bound to the enzyme as an imine with the pyridoxal phosphate, is an intermediate in the reaction.
-!- Since the selenium atom replaces oxygen in serine, the product may also be referred to as L-selenoseryl-tRNA(Sel).
-!- The abbreviation Sel has also been used for selenocysteine but Sec is preferred. |
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This specific activity occurs apparently only in bacteria; in archaea and eukaryotes, the L-selenocysteinyl-tRNA species is synthesized from O-phosphoseryl-tRNA(Ser) by Sec synthase (L-phosphoseryl-tRNA(Ser):selenophosphate seleniumtransferase). |
| PDB |
3W1J; 3W1I; 3W1H; |
| Organisms |
-Eubacteria |
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Family |
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| Links |
Enzyme (activities) 2.9.1.1
BRENDA (activities) 2.9.1.1
KEGG (pathways) 2.9.1.1
PLPMDB (PLP mutants) 2.9.1.1
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| References |
Itoh Y1, Bröcker MJ, Sekine S, Hammond G, Suetsugu S, Söll D, Yokoyama S. (2013) Decameric SelA•tRNA(Sec) ring structure reveals mechanism of bacterial selenocysteine formation. Science. 340 75-8. Gursinsky, T.; Jager, J.; Andreesen, J. R.; Sohling, B. (2000) A selDABC cluster for selenocysteine incorporation in Eubacterium acidaminophilum Arch Microbiol 174 200-12. Tormay, P.; Wilting, R.; Lottspeich, F.; Mehta, P. K.; Christen, P.; Bock, A. (1998) Bacterial selenocysteine synthase--structural and functional properties Eur J Biochem 254 655-61. Forchhammer, K.; Leinfelder, W.; Boesmiller, K.; Veprek, B.; Bock, A. (1991) Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein J Biol Chem 266 6318-23. |
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| last changed |
2019/06/20 11:47 |
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