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4.1.99.2 |
Description |
Tyrosine phenol-lyase |
Alternative names |
Beta-tyrosinase;
TPL. |
Catalyzed reaction |
L-tyrosine + H(2)O = phenol + pyruvate + NH(3). |
Cofactor |
Pyridoxal-phosphate. |
Comments |
-!- Also slowly catalyzes pyruvate formation from D-tyrosine, S-methyl- L-cysteine, L-cysteine, L-serine and D-serine. |
Prosite |
PROSITE; PDOC00667; |
PDB |
1TPL; 2TPL; |
Organisms |
-Eubacteria |
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Family |
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Links |
Enzyme (activities) 4.1.99.2
BRENDA (activities) 4.1.99.2
KEGG (pathways) 4.1.99.2
PLPMDB (PLP mutants) 4.1.99.2
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References |
Zheng RC, Tang XL, Suo H, Feng LL, Liu X, Yang J, Zheng YG (2018) Biochemical characterization of a novel tyrosine phenol-lyase from Fusobacterium nucleatum for highly efficient biosynthesis of l-DOPA Enzyme Microb Technol 112 88-93. Phillips RS, Demidkina TV, Faleev NG. (2003) Structure and mechanism of tryptophan indole-lyase and tyrosine phenol-lyase Biochim Biophys Acta 1647 167-72. Lee, S. G.; Hong, S. P.; Choi, Y. H.; Chung, Y. J.; Sung, M. H. (1997) Thermostable tyrosine phenol-lyase of Symbiobacterium sp. SC-1: gene cloning, sequence determination, and overproduction in Escherichia coli Protein Expr Purif 11 263-70. Sundararaju, B.; Antson, A. A.; Phillips, R. S.; Demidkina, T. V.; Barbolina, M. V.; Gollnick, P.; Dodson, G. G.; Wilson, K. S. (1997) The crystal structure of Citrobacter freundii tyrosine phenol-lyase complexed with 3-(4'-hydroxyphenyl)propionic acid, together with site- directed mutagenesis and kinetic analysis, demonstrates that arginine 381 is required for substrate specificity Biochemistry 36 6502-10. Iwamori, S.; Oikawa, T.; Ishiwata, K.; Makiguchi, N. (1992) Cloning and expression of the Erwinia herbicola tyrosine phenol-lyase gene in Escherichia coli Biotechnol Appl Biochem 16 77-85. Articles on 4.1.99.2 |
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last changed |
2019/06/20 12:58 |
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