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B6db activities: 4.2.1.20

4.2.1.20
Description Tryptophan synthase.
Alternative names Tryptophan synthetase;
Tryptophan desmolase.
Catalyzed reaction L-serine + 1-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H(2)O.
Cofactor Pyridoxal-phosphate.
Comments -!- Also catalyzes the conversion of indoleglycerol phosphate into indole and glyceraldehyde phosphate (alpha reaction) and of serine and indole into tryptophan and water (beta reaction).
-!- In some organisms, this enzyme is part of a multifunctional protein together with one or more components of the system for biosynthesis of tryptophan (EC 2.4.2.18, EC 4.1.1.48, EC 4.1.3.27, and EC 5.3.1.24).
Prosite PROSITE; PDOC00151; PROSITE; PDOC00152;
PDB 1TTP; 1UBS; 1BKS;
Organisms -Bacteria -Archea -Plants -Fungi
 

Family 

4.2.1.20 (57)
 
Links Enzyme (activities) 4.2.1.20
BRENDA (activities) 4.2.1.20
KEGG (pathways) 4.2.1.20
PLPMDB (PLP mutants) 4.2.1.20
 
References
 Shen H, Yang Y, Wang F, Zhang Y, Ye N, Xu S, Wang H. (2009) Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis Acta Biochim Biophys Sin (Shanghai) 41 379-88.

 Fehlner-Gardiner C, Roshick C, Carlson JH, Hughes S, Belland RJ, Caldwell HD, McClarty G. (2002) Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase J Biol Chem 277 26893-903.

 Hettwer, S.; Sterner, R. (2002) A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role J Biol Chem 277 8194-201.

 Xie, G.; Forst, C.; Bonner, C.; Jensen, R.A. (2002) Significance of two distinct types of tryptophan synthase beta chain in Bacteria, Archaea and higher plants Genome Biol 3 R-0004.

 Tang, X.F.; Ezaki, S.; Atomi, H.; Imanaka, T. (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon Eur J Biochem 267 6369-77.

 Kisaka, H.; Kisaka, M.; Lee, H. Y.; Kameya, T. (1998) Isolation of a cDNA for tryptophan synthase beta from rice and studies of its expression in a 5-methyltryptophan-resistant mutant of rice Plant Mol Biol 38 875-8.

 Lam, W.L.; Cohen, A.; Tsouluhas, D.; Doolittle, W.F. (1990) Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii Proc Natl Acad Sci U S A 87 6614-8.

 Berlyn, M.B.; Last, R.L.; Fink, G.R. (1989) A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana Proc Natl Acad Sci U S A 86 4604-8.

 Crawford, I.P.; Eberly, L. (1989) DNA sequence of the tryptophan synthase genes of Pseudomonas putida Biochimie 71 521-31.

 Hyde, C. C.; Ahmed, S. A.; Padlan, E. A.; Miles, E. W.; Davies, D. R. (1988) Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium J Biol Chem 263 17857-71.

 Zalkin H, Yanofsky C. (1982) Yeast gene TRP5: structure, function, regulation J Biol Chem 257 1491-500.

 Crawford IP, Nichols BP, Yanofsky C. (1980) Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium J Mol Biol 142 489-502.

Articles on 4.2.1.20
 
last changed 2009/06/23 09:49

B6db activities