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B6db activities: 4.3.1.18

4.3.1.18
Description D-serine ammonia-lyase.
Alternative names D-serine dehydratase;
D-serine dehydrase;
D-hydroxy amino acid dehydratase;
D-serine hydrolase;
D-serine dehydratase (deaminating);
D-serine deaminase;
D-serine hydro-lyase (deaminating).
Catalyzed reaction D-serine = pyruvate + NH(3).
Cofactor Pyridoxal-phosphate or Zn(II) ions plus pyridoxal-phosphate.
Comments -!- Also acts, slowly, on D-threonine.
-!- The reaction catalyzed probably involves initial elimination of water (hence the enzyme's original classification as EC 4.2.1.14),followed by isomerization and hydrolysis of the product with C-N bond breakage.
-!- Formerly EC 4.2.1.14.
The enzyme from E. coli is a fold-type II protein, whereas the D-ser dehydratase from S. cerevisiae is a fold-type III enzyme and employs zinc ions as cofactors, in addition to pyridoxal phosphate.
PDB 3SS7; 3SS9;
Organisms -Bacteria -Fungi -Metazoa
 

Family 

4.3.1.18_a (13) 4.3.1.18_b (11)
 
Links Enzyme (activities) 4.3.1.18
BRENDA (activities) 4.3.1.18
KEGG (pathways) 4.3.1.18
PLPMDB (PLP mutants) 4.3.1.18
 
References
 Bharath SR1, Bisht S, Savithri HS, Murthy MR. (2011) Crystal structures of open and closed forms of d-serine deaminase from Salmonella typhimurium - implications on substrate specificity and catalysis. FEBS J. 278 2879-91.

 Ito T, Hemmi H, Kataoka K, Mukai Y, Yoshimura T. (2008) A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae Biochem J 409 399-406.

 Tanaka H, Yamamoto A, Ishida T, Horiike K. (2008) D-Serine dehydratase from chicken kidney: a vertebral homologue of the cryptic enzyme from Burkholderia cepacia J Biochem 143 49-57.

 Obmolova, G.; Tepliakov, A.; Harutyunyan, E.; Wahler, G.; Schnackerz, K.D. (1990) Crystallization and preliminary X-ray studies of D-serine dehydratase from Escherichia coli J Mol Biol 214 641-2.

 McFall, E.; Runkel, L. (1983) DNA sequences of the D-serine deaminase control region and N-terminal portion of the structural gene J Bacteriol 54 1508-12.

 Dowhan W Jr, Snell EE. (1970) D-serine dehydratase from Escherichia coli. II. Analytical studies and subunit structure J Biol Chem 245 4618-28.

Articles on 4.3.1.18
 
last changed 2014/03/20 11:55

B6db activities