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B6db activities: 4.3.1.18

4.3.1.18
Description D-serine ammonia-lyase
Alternative names D-serine dehydratase;
D-serine dehydrase;
D-hydroxy amino acid dehydratase;
D-serine hydrolase;
D-serine dehydratase (deaminating);
D-serine deaminase;
D-serine hydro-lyase (deaminating).
Catalyzed reaction D-serine = pyruvate + NH(3).
Cofactor Pyridoxal-phosphate or Zn(II) ions plus pyridoxal-phosphate.
Comments Also acts, slowly, on D-threonine.
The reaction catalyzed probably involves initial elimination of water (hence the enzyme's original classification as EC 4.2.1.14),followed by isomerization and hydrolysis of the product with C-N bond breakage.
Formerly EC 4.2.1.14.
The enzyme from E. coli is a fold-type II protein, whereas the D-ser dehydratase from S. cerevisiae is a fold-type III enzyme and employs zinc ions as cofactors, in addition to pyridoxal phosphate.
PDB 3SS7; 3SS9;
Organisms -Eubacteria -Fungi -Metazoa
 

Family 

4.3.1.18_a (14) 4.3.1.18_b (11)
 
Links Enzyme (activities) 4.3.1.18
BRENDA (activities) 4.3.1.18
KEGG (pathways) 4.3.1.18
PLPMDB (PLP mutants) 4.3.1.18
 
References
 Li, G.; Lu, C.D. (2016) The Cryptic dsdA Gene Encodes a Functional D-Serine Dehydratase in Pseudomonas aeruginosa PAO1. Curr Microbiol. 72 788-94.

 Bharath SR, Bisht S, Savithri HS, Murthy M.R. (2011) Crystal structures of open and closed forms of d-serine deaminase from Salmonella typhimurium - implications on substrate specificity and catalysis. FEBS J. 278 2879-91.

 Ito T, Hemmi H, Kataoka K, Mukai Y, Yoshimura T. (2008) A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae Biochem J 409 399-406.

 Tanaka H, Yamamoto A, Ishida T, Horiike K. (2008) D-Serine dehydratase from chicken kidney: a vertebral homologue of the cryptic enzyme from Burkholderia cepacia J Biochem 143 49-57.

 Obmolova, G.; Tepliakov, A.; Harutyunyan, E.; Wahler, G.; Schnackerz, K.D. (1990) Crystallization and preliminary X-ray studies of D-serine dehydratase from Escherichia coli J Mol Biol 214 641-2.

 McFall, E.; Runkel, L. (1983) DNA sequences of the D-serine deaminase control region and N-terminal portion of the structural gene J Bacteriol 54 1508-12.

 Dowhan W Jr, Snell EE. (1970) D-serine dehydratase from Escherichia coli. II. Analytical studies and subunit structure J Biol Chem 245 4618-28.

Articles on 4.3.1.18
 
last changed 2019/06/20 13:01

B6db activities