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B6db activities: 4.4.1.9

4.4.1.9
Description L-3-cyanoalanine synthase.
Alternative names Beta-cyanoalanine synthase;
Beta-cyano-L-alanine synthase.
Catalyzed reaction L-cysteine + cyanide = H(2)S + L-3-cyanoalanine.
Cofactor Pyridoxal-phosphate.
Comments Enzymes with this activity are strictly related to Cysteine Synthase (EC 2.5.1.47) both from a mechanistic and from a phylogenetic viewpoint.

The family corresponding to this activity is composed only of plant enzymes, which appear to be mitochondrial isozymes of Cysteine Synthase.

One cyanoalanine synthase from bacteria (Bacillus stearothermophilus) has also been cloned and characterized, but this enzyme is in fact tenfold more active as a cysteine synthase, and has been included in the 2.5.1.47 family.

Organisms -Bacteria -Plants
 

Family 

4.4.1.9 (6)
 
Links Enzyme (activities) 4.4.1.9
BRENDA (activities) 4.4.1.9
KEGG (pathways) 4.4.1.9
PLPMDB (PLP mutants) 4.4.1.9
 
References
 Omura H, Kuroda M, Kobayashi M, Shimizu S, Yoshida T, Nagasawa T. (2003) Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming beta-cyano-L-alanine J Biosci Bioeng 95 470-5.

 Maruyama, A.; Saito, K.; Ishizawa, K. (2001) Beta-cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation. Plant Mol Biol 51 985-93.

 Hatzfeld, Y.; Maruyama, A.; Schmidt, A.; Noji, M.; Ishizawa, K.; Saito, K. (2000) beta-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis Plant Physiol 123 1163-71.

 Warrilow, A. G.; Hawkesford, M. J. (2000) Cysteine synthase (O-acetylserine (thiol) lyase) substrate specificities classify the mitochondrial isoform as a cyanoalanine synthase J Exp Bot 51 985-93.

Articles on 4.4.1.9
 
last changed 2009/07/01 13:10

B6db activities