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B6db activities: 5.1.1.13

5.1.1.13
Description Aspartate racemase
Catalyzed reaction L-aspartate = D-aspartate
Cofactor Pyridoxal-phosphate or no cofactor
Known aspartate racemases from eubacteria are not PLP-dependent. In Archaea, PLP-dependent enzymes have been isolated but not characterized at the molecular level.

Aspartate racemases from metazoa have emerged (apparently multiple times) from ancestral serine racemases (fold-type II).They have been grouped here in two related but phylogenetically distinct families.

A mammalian protein with Fold-type I and related to aspartate aminotransferase had been once attributed an Asp racemase function, but this result has been subsequently questioned.

Prosite PDOC00714
PDB 5YBW;
Organisms -Archea -Metazoa
 

Family 

 
Links Enzyme (activities) 5.1.1.13
BRENDA (activities) 5.1.1.13
KEGG (pathways) 5.1.1.13
PLPMDB (PLP mutants) 5.1.1.13
 
References
 Uda K, Ishizuka N, Edashige Y, Kikuchi A, Radkov AD, Moe LA (2019) Cloning and characterization of a novel aspartate/glutamate racemase from the acorn worm Saccoglossus kowalevskii Comp Biochem Physiol B Biochem Mol Biol 232 :87-92.

 Mizobuchi T, Nonaka R, Yoshimura M, Abe K, Takahashi S, Kera Y, Goto M (2017) Crystal structure of a pyridoxal 5'-phosphate-dependent aspartate racemase derived from the bivalve mollusc Scapharca broughtonii Acta Crystallogr F Struct Biol Commun 73 651-656.

 Uda K, Abe K, Dehara Y, Mizobata K, Sogawa N, Akagi Y, Saigan M, Radkov AD, Moe LA. (2016) Distribution and evolution of the serine/aspartate racemase family in invertebrates Amino Acids 48 387-402.

 Tanaka-Hayashi A, Hayashi S, Inoue R, Ito T, Konno K, Yoshida T, Watanabe M, Yoshimura T, Mori H. (2015) Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1 (Got1l1): a putative aspartate racemase? Amino Acids 47 79-86.

 Kim PM, Duan X, Huang AS, Liu CY, Ming GL, Song H, Snyder SH (2010) Aspartate racemase, generating neuronal D-aspartate, regulates adult neurogenesis Proc Natl Acad Sci U S A 107 3175-9.

 Abe K, Takahashi S, Muroki Y, Kera Y, Yamada RH. (2006) Cloning and expression of the pyridoxal 5'-phosphate-dependent aspartate racemase gene from the bivalve mollusk Scapharca broughtonii and characterization of the recombinant enzyme J Biochem 139 235-44.

 Shibata, K.; Watanabe, T.; Yoshikawa, H.; Abe, K.; Takahashi, S.; Kera, Y.; Yamada, R. (2003) Purification and characterization of aspartate racemase from the bivalve mollusk Scapharca broughtonii Comp Biochem Physiol B Biochem Mol Biol 134 307-314.

 Long, Z.; Lee, J. A.; Okamoto, T.; Sekine, M.; Nimura, N.; Imai, K.; Yohda, M.; Maruyama, T.; Sumi, M.; Kamo, N.; Yamagishi, A.; Oshima, T.; Homma, H. (2001) Occurrence of D-Amino Acids and a pyridoxal 5'-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum Biochem Biophys Res Commun 281 317-21.

Articles on 5.1.1.13
 
last changed 2019/06/20 13:31

B6db activities