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B6db activities: 5.4.3.8

5.4.3.8
Description Glutamate-1-semialdehyde 2,1-aminomutase.
Alternative names Glutamate-1-semialdehyde aminotransferase.
Catalyzed reaction (S)-4-amino-5-oxopentanoate = 5-aminolevulinate.
Cofactor Pyridoxal-phosphate or Pyridoxamine-phosphate.
Glutamate-1-semialdehyde 2,1-aminomutase is the second enzyme in the tetrapyrrole biosynthesis pathway found in most bacteria, in archaea and in plants (the first enzyme in the pathway is a most unusual glutamyl-tRNA reductase).
The enzyme has therefore paramount importance in the biosynthesis of compounds such as heme and chlorophyll.
Prosite PROSITE; PDOC00519;
PDB 2GSA; 3GSB; 4GSA;
Organisms -Eubacteria -Archea -Plants
 

Family 

5.4.3.8 (44)
 
Links Enzyme (activities) 5.4.3.8
BRENDA (activities) 5.4.3.8
KEGG (pathways) 5.4.3.8
PLPMDB (PLP mutants) 5.4.3.8
 
References
 Schulze JO, Schubert WD, Moser J, Jahn D, Heinz DW. (2006) Evolutionary relationship between initial enzymes of tetrapyrrole biosynthesis J Mol Biol 358 1212-20.

 Atteia A, van Lis R, Beale SI. (2005) Enzymes of the heme biosynthetic pathway in the nonphotosynthetic alga Polytomella sp. Eukaryot Cell 4 2087-97.

 Tsang, E.W.; Hu Z.; Chang, Q.; McGregor, D.I.; Keller, W.A. (2003) Expression of a Brassica napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein Protein Expr Purif 29 193-201..

 Hennig, M.; Grimm, B.; Contestabile, R.; John, R. A.; Jansonius, J. N. (1997) Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity Proc Natl Acad Sci U S A 94 4866-71.

 Palmieri, G.; Di Palo, M.; Scaloni, A.; Orru, S.; Marino, G.; Sannia, G. (1996) Glutamate-1-semialdehyde aminotransferase from Sulfolobus solfataricus Biochem J 320 541-5.

 Murakami, K.; Hashimoto, Y.; Murooka, Y. (1993) Cloning and characterization of the gene encoding glutamate 1-semialdehyde 2,1-aminomutase, which is involved in delta-aminolevulinic acid synthesis in Propionibacterium freudenreichii Appl Environ Microbiol 59 347-50.

 Murakami, K.; Korbsrisate, S.; Asahara, N.; Hashimoto, Y.; Murooka, Y. (1993) Cloning and characterization of the glutamate 1-semialdehyde aminomutase gene from Xanthomonas campestris pv. phaseoli Appl Microbiol Biotechnol 38 502-6..

 Sangwan, I.; O'Brian, M. R. (1993) Expression of the soybean (Glycine max) glutamate 1-semialdehyde aminotransferase gene in symbiotic root nodules Plant Physiol 102 829-34.

 Ilag, L. L.; Jahn, D.; Eggertsson, G.; Soll, D. (1991) The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase J Bacteriol 173 3408-13.

 Elliott, T.; Avissar, Y. J.; Rhie, G. E.; Beale, S. I. (1990) Cloning and sequence of the Salmonella typhimurium hemL gene and identification of the missing enzyme in hemL mutants as glutamate-1-semialdehyde aminotransferase J Bacteriol 172 7071-84.

 Grimm, B. (1990) Primary structure of a key enzyme in plant tetrapyrrole synthesis: glutamate 1-semialdehyde aminotransferase Proc Natl Acad Sci U S A 87 4169-73..

Articles on 5.4.3.8
 
last changed 2010/02/03 19:19

B6db activities