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B6db activities: 1.4.4.2

1.4.4.2
Description Glycine dehydrogenase (decarboxylating).
Alternative names Glycine decarboxylase;
glycine cleavage system P-protein;
P-protein;
glycine-cleavage complex;
glycine:lipoylprotein oxidoreductase (decarboxylating and acceptor-aminomethylating);
protein P1
Catalyzed reaction glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO(2).
Cofactor Pyridoxal-phosphate.
Comments -!- Lipoamide can also act as acceptor.
-!- A component, with EC 2.1.2.10, aminomethyltransferase and EC 1.8.1.4, dihydrolipoyl dehydrogenanse, of the glycine cleavage system, previously known as glycine synthase. The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC 1.4.4.2), the T protein (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H protein.
The P protein from archaea and from certain (gram positive) bacteria is formed by two subunits. See for example: gi|14591729|ref|NP_143817.1| glycine dehydrogenase subunit 1 [Pyrococcus horikoshii] gi|14591728|ref|NP_143816.1| glycine dehydrogenase subunit 2 [Pyrococcus horikoshii]. Even though these subunits are phylogenetically related, only one apparently retains the ability to bind PLP (subunit 2 in the example above). In other eubacteria and in eukarya, a single polypeptide contains domains corresponding to the subunits 1 and 2.
Diseases Nonketotic hyperglycinemia type II
PDB 1WYU; 4LDH; 4LHC;
Organisms -All -Eubacteria -Archea -Plants -Fungi -Metazoa -Human
 

Family 

1.4.4.2 (30)
 
Links Enzyme (activities) 1.4.4.2
BRENDA (activities) 1.4.4.2
KEGG (pathways) 1.4.4.2
PLPMDB (PLP mutants) 1.4.4.2
 
References
 Hasse D.; Mikkat S.; Thrun H.A.; Hagemann, M.; Bauwe, H. (2007) Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803. FEBS Lett 581 1297-301.

 Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N. (2005) Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia EMBO J 24 1523-1536.

 Van Cauwenberghe, O.R.; Makhmoudova, A.; McLean, M.D.; Clark, S.M.; Shelp, B.J. (2002) Plant pyruvate-dependent gamma-aminobutyrate transaminase:Identification of an Arabidopsis cDNA and its expression in Escherichia coli Can J Bot 80 933-41.

 Douce, R.; Bourguignon, J.; Neuburger, M.; Rebeille, F. (2001) The glycine decarboxylase system: a fascinating complex Trends Plant Sci 6 167-76.

 Sinclair, D.A.; Hong, S.P.; Dawes, I.W. (1996) Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae Mol Microbiol 19 611-23.

 Turner, S.R.; Ireland, R.; Rawsthorne, S. (1992) Cloning and characterization of the P subunit of glycine decarboxylase from pea (Pisum sativum) J Biol Chem 267 5355-60.

 Fujiwara, K.; Okamura-Ikeda, K.; Motokawa, Y. (1987) Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system Biochem Biophys Res Commun 149 621-7.

Articles on 1.4.4.2
 
last changed 2014/07/10 20:08

B6db activities