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B6db activities:
Description 8-amino-7-oxononanoate synthase
Alternative names AONS;
8-amino-7-ketopelargonate synthase;
7-keto-8-amino-pelargonic acid synthetase.;
7-KAP synthetase.
KAPA synthase;
alpha-Oxoamine Synthase (ambiguous;
BioF (gene name)
Catalyzed reaction 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO(2).
Cofactor Pyridoxal-phosphate.
Comments The known enzymes with this activity show a strict phylogenetic relationship to glycine acetyltransferases (EC
Indeed, some of these enzymes possess a broad substrate specificity, performing also the glycine acetyltransferase reaction.
Prosite PROSITE; PDOC00518;
PDB 1DJE; 1DJ9; 1BS0;
Organisms -Eubacteria -Archea -Plants -Fungi

Family (37)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Ren X, Wang X, Shi H, Zhang X, Chen Z, Malhi KK, Ding C, Yu S (2018) Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence Sci Rep 8 14645.

 Kubota T, Izumi Y. (2012) Detection and characterization of a thermophilic biotin biosynthetic enzyme, 7-keto-8-aminopelargonic acid synthase, from various thermophiles. Biosci Biotechnol Biochem 76 685-90.

 Kubota T, Shimono J, Kanameda C, Izumi Y. (2007) The first Thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase: Cloning and overexpression of the gene from an extreme thermophile, Thermus thermophilus, and characterization of its gene product Biosci Biotechnol Biochem 23 3033-3040.

 Bhor VM, Dev S, Vasanthakumar GR, Kumar P, Sinha S, Surolia A. (2006) Broad substrate stereospecificity of the Mycobacterium tuberculosis 7-keto-8-aminopelargonic acid synthase: Spectroscopic and kinetic studies J Biol Chem 281 25076-88.

 Pinon V, Ravanel S, Douce R, Alban C. (2005) Biotin synthesis in plants. The first committed step of the pathway is catalyzed by a cytosolic 7-keto-8-aminopelargonic acid synthase Plant Physiol 139 1666-76.

 Alexeev, D.; Alexeeva, M.; Baxter, R. L.; Campopiano, D. J.; Webster, S. P.; Sawyer, L. (1998) The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme J Mol Biol 284 401-19.

 Ploux, O.; Marquet, A. (1992) The 8-amino-7-oxopelargonate synthase from Bacillus sphaericus. Purification and preliminary characterization of the cloned enzyme overproduced in Escherichia coli Biochem J 283 327-31.

Articles on
last changed 2019/06/20 13:26

B6db activities