B6db activities : 2_5_1

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	2.5.1.113
Description	[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase
Alternative names	O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase; CysM.
Catalyzed reaction	O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
Cofactor	Pyridoxal-phosphate.
Comments	A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
PDB	3DKI; 3DWG;
Organisms	-Eubacteria
Family	2.5.1.113 (0)

Links	Enzyme (activities) 2.5.1.113 BRENDA (activities) 2.5.1.113 KEGG (pathways) 2.5.1.113 PLPMDB (PLP mutants) 2.5.1.113

References	Agren D, Schnell R, Oehlmann W, Singh M, Schneider G. (2008) Cysteine Synthase (CysM) of Mycobacterium tuberculosis Is an O-Phosphoserine Sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria. J Biol Chem 283 31567-74. Jurgenson CT1, Burns KE, Begley TP, Ealick SE. (2008) Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis. Biochemistry 47 10354-64. O'Leary SE, Jurgenson CT, Begley TP, Ealick SE. (2008) O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis. Biochemistry 47 11606-15. Articles on 2.5.1.113

last changed	2019/06/21 13:02

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