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2.5.1.48 |
| Description |
Cystathionine gamma-synthase |
| Alternative names |
O-succinylhomoserine (thiol)-lyase;
Homoserine transsuccinylase.;
Cystathionine synthase;
MetB. |
| Catalyzed reaction |
O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate. |
| Cofactor |
Pyridoxal-phosphate. |
| Comments |
-!- Can also use hydrogen sulfide and methanethiol as substrates producing homocysteine and methionine respectively.
-!- In the absence of thiol, can also catalyse beta,gamma-elimination to form 2-oxobutanoate, succinate and ammonia.
The plant enzyme can also use O-phosphohomoserine as a substrate.
Formerly EC 4.2.99.9. |
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Subfamily 2.5.1.48a includes enzymes from gram-negative bacteria; subfamily b encompasses enzymes from plants (plastidial proteins) and cyanobacteria. |
| Prosite |
PROSITE; PDOC00677; |
| PDB |
1I48; 1I43; 1I41; 1CS1; 1QGN; |
| Organisms |
-Eubacteria -Archea -Plants -Fungi |
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Family |
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| Links |
Enzyme (activities) 2.5.1.48
BRENDA (activities) 2.5.1.48
KEGG (pathways) 2.5.1.48
PLPMDB (PLP mutants) 2.5.1.48
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| References |
Sato D, Shiba T, Mizuno S, Kawamura A, Hanada S, Yamada T, Shinozaki M, Yanagitani M, Tamura T, Inagaki K, Harada S (2017) The hyperthermophilic cystathionine γ-synthase from the aerobic crenarchaeon Sulfolobus tokodaii: expression, purification, crystallization and structural insights Acta Crystallogr F Struct Biol Commun 73 152-158. . Kong Y, Wu D, Bai H, Han C, Chen J, Chen L, Hu L, Jiang H, Shen X. (2008) Enzymatic characterization and inhibitor discovery of a new cystathionine {gamma}-synthase from Helicobacter pylori J Biochem 143 59-68. Steegborn, C.; Messerschmidt, A.; Laber, B.; Streber, W.; Huber, R.; Clausen, T. (1999) The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity J Mol Biol 290 983-96. Ravanel, S.; Gakiere, B.; Job, D.; Douce, R. (1998) Cystathionine gamma-synthase from Arabidopsis thaliana: purification and biochemical characterization of the recombinant enzyme overexpressed in Escherichia coli Biochem J 331 639-48. Wahl, M. C.; Huber, R.; Prade, L.; Marinkovic, S.; Messerschmidt, A.; Clausen, T. (1997) Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli FEBS Lett 414 492-6. |
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| last changed |
2010/01/27 18:06 |
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