|
|
2.5.1.49 |
Description |
O-acetylhomoserine (thiol)-lyase. |
Alternative names |
O-acetylhomoserine aminocarboxypropyltransferase; Methionine synthase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase. |
Catalyzed reaction |
O-acetyl-L-homoserine + methanethiol = L-methionine + acetate. |
Cofactor |
Pyridoxal-phosphate |
Comments |
-!- Also reacts with other thiols or H(2)S, producing homocysteine or thioethers. -!- The name methionine synthase is more commonly applied to EC 2.1.1.13. -!- An enzyme from baker's yeast also catalyzes the reaction of EC 2.5.1.47, more slowly. |
|
Formerly 4.2.99.10 |
Prosite |
PROSITE; PDOC00677; |
Organisms |
-Eubacteria -Archea -Fungi |
| |
Family |
|
| |
Links |
Enzyme (activities) 2.5.1.49
BRENDA (activities) 2.5.1.49
KEGG (pathways) 2.5.1.49
PLPMDB (PLP mutants) 2.5.1.49
|
| |
References |
Shimizu H, Yamagata S, Masui R, Inoue Y, Shibata T, Yokoyama S, Kuramitsu S, Iwama T.
(2001) Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product
Biochim Biophys Acta 1549 1-72. Yamagata, S.; Ichioka, K.; Goto, K.; Mizuno, Y; Iwama, T.
(2001) Occurrence of transsulfuration in synthesis of L-homocysteine in an extremely thermophilic bacterium, Thermus thermophilus HB8
J Bacteriol 183 2086-92. Bourhy, P.; Martel, A.; Margarita, D.; Saint Girons, I.; Belfaiza, J.
(1997) Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited J Bacteriol 179 4396-4398. Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R. (1994) Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine.O-acetylhomoserine sulfhydrylase of the yeast Appl Microbiol Biotechnol 42 92-99. Brzywczy, J.; Yamagata, S.; Paszewski, A. (1993) Comparative studies on O-acetylhomoserine sulfhydrylase: physiological role and characterization of the Aspergillus nidulans enzyme Acta Biochim Pol 40 421-8. Articles on 2.5.1.49 |
| |
last changed |
2010/01/28 11:07 |
|