B6db activities : 2_5_1

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	2.5.1.49
Description	O-acetylhomoserine (thiol)-lyase
Alternative names	O-acetylhomoserine aminocarboxypropyltransferase; Methionine synthase (ambiguous); O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase; Homocysteine synthase;
Catalyzed reaction	O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.
Cofactor	Pyridoxal-phosphate
Comments	-!- Also reacts with other thiols or H(2)S, producing homocysteine or thioethers. -!- The name methionine synthase is more commonly applied to EC 2.1.1.13. -!- An enzyme from baker's yeast also catalyzes the reaction of EC 2.5.1.47, more slowly.
	Formerly 4.2.99.10
Prosite	PROSITE; PDOC00677;
PDB	2CTZ; 4KAM;
Organisms	-Eubacteria -Archea -Fungi
Family	2.5.1.49 (30)

Links	Enzyme (activities) 2.5.1.49 BRENDA (activities) 2.5.1.49 KEGG (pathways) 2.5.1.49 PLPMDB (PLP mutants) 2.5.1.49

References	Kulikova VV, Revtovich SV, Bazhulina NP, Anufrieva NV, Kotlov MI, Koval VS, Morozova EA, Hayashi H, Belyi YF, Demidkina TV (2019) Identification of O-acetylhomoserine sulfhydrylase, a putative enzyme responsible for methionine biosynthesis in Clostridioides difficile: Gene cloning and biochemical characterizations IUBMB Life 71 1815-1823. Allen KD, Miller DV, Rauch BJ, Perona JJ, White RH (2015) Homocysteine is biosynthesized from aspartate semialdehyde and hydrogen sulfide in methanogenic archaea Biochemistry 54 3129-32. Omura H, Ikemoto M, Kobayashi M, Shimizu S, Yoshida T, Nagasawa T (2003) Purification, characterization and gene cloning of thermostable O-acetyl-L-homoserine sulfhydrylase forming gamma-cyano-alpha-aminobutyric acid J Biosci Bioeng 96 53-8. Shimizu H, Yamagata S, Masui R, Inoue Y, Shibata T, Yokoyama S, Kuramitsu S, Iwama T. (2001) Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product Biochim Biophys Acta 1549 1-72. Yamagata, S.; Ichioka, K.; Goto, K.; Mizuno, Y; Iwama, T. (2001) Occurrence of transsulfuration in synthesis of L-homocysteine in an extremely thermophilic bacterium, Thermus thermophilus HB8 J Bacteriol 183 2086-92. Bourhy, P.; Martel, A.; Margarita, D.; Saint Girons, I.; Belfaiza, J. (1997) Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited J Bacteriol 179 4396-4398. Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R. (1994) Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine.O-acetylhomoserine sulfhydrylase of the yeast Appl Microbiol Biotechnol 42 92-99. Brzywczy, J.; Yamagata, S.; Paszewski, A. (1993) Comparative studies on O-acetylhomoserine sulfhydrylase: physiological role and characterization of the Aspergillus nidulans enzyme Acta Biochim Pol 40 421-8. Articles on 2.5.1.49

last changed	2019/08/28 11:40

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