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Description GDP-perosamine synthase
Alternative names perosamine synthetase;
Catalyzed reaction GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate <=> GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate.
Cofactor Pyridoxal-phosphate.
Comments D-perosamine is one of several dideoxy sugars found in the O-specific polysaccharide of the lipopolysaccharide component of the outer membrane of Gram-negative bacteria. The enzyme catalyzes the final step in GDP-perosamine synthesis.
In bacteria,D-perosamine is used for the glycosylation of products such as antibiotics and lipopolysaccharides.
Perosamine synthase occurs in a wide variety of microorganisms, including pathogens, suggesting that the enzyme performs a function crucial for the normal life cycle and/or virulence of many species.
Organisms -Eubacteria

Family (0)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Cook PD, Holden HM. (2008) GDP-perosamine synthase: structural analysis and production of a novel trideoxysugar Biochemistry 47 2833-40.

 Zhao G, Liu J, Liu X, Chen M, Zhang H, Wang PG. (2007) Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro Biochem Biophys Res Commun 363 525-30.

 Muller-Dieckmann, Hans-Joachim ; et al. (2003) Crystals and structures of perosamine synthase homologs United States Patent Application 20030101005 .

 Albermann, C.; Piepersberg, W. (2001) Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine Glycobiology 11 655-61.

 Godfroid, F., Taminiau, B., Danese, I., Denoel, P., Tibor, A., Weynants, V., Cloeckaert, A., Godfroid, J.; Letesson J.J. (1998) Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages Infect. Immun. 66 5485-5493.

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last changed 2014/02/19 11:38

B6db activities