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2.6.1.33 |
| Description |
dTDP-4-amino-4,6-dideoxy-D-glucose aminotransferase |
| Alternative names |
Thymidine diphospho-4-keto-6-deoxy-D-glucose transaminase;
Thymidine diphospho-4-keto-6-deoxy-D-glucose-glutamic transaminase;
TDP-4-keto-6-deoxy-D-glucose transaminase;
dTDP-4-keto-6-deoxy-D-glucose aminotransferase;
Thymidine diphospho-4-amino-4,6-dideoxyglucose transaminase;
CalS13 (gene name)
D-viosamine synthase;
VioA (gene name);
DesI (gene name);
GerB (gene name);
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| Catalyzed reaction |
dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate. |
| Cofactor |
Pyridoxal-phosphate. |
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The enzyme from Micromonospora echinospora, CalS13 (upon which subfamily a is based), is involved in calicheamicin biosynthesis, where it catalyzes the reaction in the direction opposite to the one shown above.
The enterobacterial enzyme VioA (subfamily b) is responsible for the synthesis of D-Viosamine (4-amino-4,6-dideoxy-D-glucose), whose derivatives, such as 4-(N-acetylglycyl)amino-4,6-dideoxy-D-glucose, are component of O-antigen variants.
In Streptomyces, the same reaction is exploited in metabolic pathways leading to the production of macrolides. |
| PDB |
4ZAS; 2PO3; 3JU7; |
| Organisms |
-Eubacteria |
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Family |
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| Links |
Enzyme (activities) 2.6.1.33
BRENDA (activities) 2.6.1.33
KEGG (pathways) 2.6.1.33
PLPMDB (PLP mutants) 2.6.1.33
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| References |
Brown HA, Vinogradov E, Gilbert M, Holden HM (2018) The Mycobacterium tuberculosis Complex has a Pathway for the
Biosynthesis of 4-Formamido-4,6-Dideoxy-D-Glucose Protein Science 27 1491-1497. Wang F, Singh S, Xu W, Helmich KE, Miller MD, Cao H, Bingman CA, Thorson JS, Phillips GN Jr.
(2015) Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases. ACS chemical biology. 10 2048-56. Singh S, Peltier-Pain P, Tonelli M, Thorson JS
(2014) A general NMR-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways Org Lett 16 3220-3. Dong S, McPherson SA, Wang Y, Li M, Wang P, Turnbough CL Jr, Pritchard DG. (2010) Characterization of the enzymes encoded by the anthrose biosynthetic operon of Bacillus anthracis
J Bacteriol 192 5053-62. Burgie ES, Holden HM. (2007) Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine
Biochemistry 46 8999-9006. Chung YS, Kim DH, Seo WM, Lee HC, Liou K, Oh TJ, Sohng JK. (2007) Enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-D-glucose using GerB (dTDP-4-keto-6-deoxy-D-glucose aminotransferase)
Carbohydr Res 342 1412-8. Wang Y, Xu Y, Perepelov AV, Qi Y, Knirel YA, Wang L, Feng L. (2007) Biochemical characterization of dTDP-D-Qui4N and dTDP-D-Qui4NAc biosynthetic pathways in Shigella dysenteriae type 7 and Escherichia coli O7
J Bacteriol 189 8626-35. |
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| last changed |
2019/06/24 12:38 |
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