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2.6.1.36 |
| Description |
L-lysine aminotransferase. |
| Alternative names |
L-Lysine 6-aminotransferase;
Lysine-epsilon aminotransferase;
L-Lysine 6-transaminase.
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| Catalyzed reaction |
L-lysine + 2-oxoglutarate = 2-aminoadipate 6-semialdehyde + L-glutamate. |
| Cofactor |
Pyridoxal-phosphate. |
| Comments |
-!- The product (allysine) is converted into the intramolecularly dehydrated form, 1-piperideine 6-carboxylate. |
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The enzyme is involved in L-lysine catabolism. In actinomycetes, this enzyme is specific to the first step of the beta-lactam antibiotic biosynthetic pathway. An enzyme from Sphingomonas paucimobilis has been cloned (see Patel et al., 2000) but the sequence is not available from current primary databases. |
| PDB |
2CJH; 2CJD; 2CIN; |
| Organisms |
-Eubacteria -Fungi |
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Family |
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| Links |
Enzyme (activities) 2.6.1.36
BRENDA (activities) 2.6.1.36
KEGG (pathways) 2.6.1.36
PLPMDB (PLP mutants) 2.6.1.36
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| References |
Mani Tripathi S, Ramachandran R.
(2006) Overexpression, purification and crystallization of lysine epsilon-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv Acta Crystallogr Sect F Struct Biol Cryst Commun 62 572-5. Mani Tripathi S, Ramachandran R.
(2006) Direct evidence for a glutamate switch necessary for substrate recognition: crystal structures of lysine epsilon-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv J Mol Biol 362 877-86. Fujii, T.; Narita, T.; Agematu, H.; Agata, N.; Isshiki, K.
(2000) Characterization of L-lysine 6-aminotransferase and its structural gene from Flavobacterium lutescens IFO3084 J Biochem (Tokyo) 128 391-7. Patel, R.N.
et al. (2000) Biocatalytic preparation of a chiral synthon for a vasopeptidase inhibitor: enzymatic conversion of N-2-[N-phenylmethoxy)carbonyl] L-homocysteinyl]-L-lysine (1-> 1 ')-disulfide to [4S-(4I,7I,10aJ)] 1-octahydro-5-oxo-4-[phenylmethoxy)carbonyl]amino]-7H-pyrido-[2,1-b] [1,3]thiazepine-7-carboxylic acid methyl ester by a novel L-lysine epsilon-aminotransferase
Enzyme Microb Tech 27 376-389 . Coque, J. J.; Liras, P.; Laiz, L.; Martin, J. F. (1991) A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans J Bacteriol 173 6258-64. Tobin, M.B.; Kovacevic, S.; Madduri, K.; Hoskins, J.A.; Skatrud, P.L.; Vining, L.C.; Stuttard, C.; Miller, J.R. (1991) Localization of the lysine epsilon-aminotransferase (lat) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (pcbAB) genes from Streptomyces clavuligerus and production of lysine epsilon-aminotransferase activity in Escherichia coli J Bacteriol 173 6223-9. |
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| last changed |
2010/03/11 16:16 |
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