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B6db activities:
Description D-4-hydroxyphenylglycine aminotransferase
Alternative names D-phenylglycine aminotransferase
Catalyzed reaction D-4-hydroxyphenylglycine + 2-oxoglutarate = 4-hydroxyphenylglyoxylate + L-glutamate.
Cofactor Pyridoxal-phosphate.
The enzyme from Pseudomonas strutzeri accepts as amino group donor only D-4-hydroxyphenylglycine or D-phenylglycine, whereas 2-oxoglutarate is the exclusive amino group acceptor and is converted into L-glutamate.
Organisms -Eubacteria

Family (0)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Walton CJ, Thiebaut F, Brunzelle JS, Couture JF, Chica RA (2018) Structural determinants of the stereo-inverting activity of Pseudomonas stutzeri D-phenylglycine aminotransferase Biochemistry 57 5437-5446.

 Chantarasiri A, Meevootisom V, Isarangkul D, Wiyakrutta S. (2012) Effective improvement of D-phenylglycine aminotransferase solubility by protein crystal contact engineering J Mol Microbiol Biotechnol 22 147-55.

 Müller U, van Assema F, Gunsior M, Orf S, Kremer S, Schipper D, Wagemans A, Townsend CA, Sonke T, Bovenberg R, Wubbolts M. (2006) Metabolic engineering of the E. coli L-phenylalanine pathway for the production of D-phenylglycine (D-Phg) Metab Eng 8 196-208.

 Wiyakrutta S, Meevootisom V. (1997) A stereo-inverting D-phenylglycine aminotransferase from Pseudomonas stutzeri ST-201: purification, characterization and application for D-phenylglycine synthesis J Biotechnol 55 193-203.

 Van den Tweel, W.J.J.; Smits, J.P.; Ogg, R.L.H.P.; de Bont, J.A.M. (1988) The involvement of an enantioselctive transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine in Pseudomonas putida LW-4 Appl Microbiol Biotechnol 29 224-230.

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last changed 2019/06/20 11:22

B6db activities