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B6db activities: 2.6.1.76

2.6.1.76
Description Diaminobutyrate-2-oxoglutarate transaminase
Alternative names L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase;
2,4-diaminobutyrate 4-aminotransferase;
Diaminobutyrate transaminase;
DABA aminotransferase;
ectB (gene name);
pvdH (gene name);
Catalyzed reaction L-2,4-diaminobutyrate + 2-oxoglutarate = L-glutamate + L-aspartic 4-semialdehyde.
Cofactor Pyridoxal-phosphate; Potassium.
Comments -!- Involved in the formation of 1,3-diaminopropane in Haemophilus influenzae and Acinetobacter baumannii.
-!- A product of the ddc gene that also encodes L-2,4-diaminobutyrate decarboxylase in Acinetobacter baumannii.
-!- Differs from EC 2.6.1.46, diaminobutyrate-pyruvate transaminase which has pyruvate as the amino-group acceptor.

This is the first enzyme in the ectoine biosynthesis pathway, the other enzymes involved being EC 2.3.1.178 and EC 4.2.1.108.
Prosite PROSITE; PDOC00519;
PDB 6RL5;
Organisms -Eubacteria
 

Family 

2.6.1.76 (30)
 
Links Enzyme (activities) 2.6.1.76
BRENDA (activities) 2.6.1.76
KEGG (pathways) 2.6.1.76
PLPMDB (PLP mutants) 2.6.1.76
 
References
 Hillier HT, Altermark B, Leiros I (2020) The crystal structure of the tetrameric DABA-aminotransferase EctB, a rate-limiting enzyme in the ectoine biosynthesis pathway FEBS J in press.

 Anburajan L, Meena B, Vinithkumar NV, Kirubagaran R, Dharani G (2019) Functional characterization of a major compatible solute in Deep Sea halophilic eubacteria of active volcanic Barren Island, Andaman and Nicobar Islands, India Infect Genet Evol 73 261-265.

 Richter AA, Mais C-N, Czech L, Geyer K, Hoeppner A, Smits Sander HJ, Erb TJ, Bange G, Bremer E (2019) Biosynthesis of the Stress-Protectant and Chemical Chaperon Ectoine: Biochemistry of the Transaminase EctB Front Microbiol 10 2811.

 Lee J, Sperandio V, Frantz DE, Longgood J, Camilli A, Phillips MA, Michael AJ. (2009) An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae J Biol Chem 284 9899-907.

 Vandenende C.S.; Vlasschaert, M.; Seah, S.Y. (2004) Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1 J Bacteriol 186 5596-602.

 Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y. (1999) Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata J Bacteriol 181 91-9.

 Ikai, H.; Yamamoto, S. (1998) Two genes involved in the 1,3-diaminopropane production pathway in Haemophilus influenzae Biol Pharm Bull 21 170-3.

 Ikai, H.; Yamamoto, S. (1997) Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2- ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii J Bacteriol 179 5118-25.

Articles on 2.6.1.76
 
last changed 2020/03/01 11:13

B6db activities