Activities | Families | Sequences | Fold types | References | Help
B6db activities:
Description Glutamate--prephenate aminotransferase
Alternative names prephenate transaminase (ambiguous);
PAT (ambiguous);
L-glutamate:prephenate aminotransferase;
L-arogenate:2-oxoglutarate aminotransferase.
Catalyzed reaction L-arogenate + 2-oxoglutarate = prephenate + L-glutamate
Cofactor Pyridoxal-phosphate.
Comments A pyridoxal-phosphate protein. Aspartate can also act as the amino donor, but more slowly (cf. EC, aspartate--prephenate aminotransferase).
The enzyme from higher plants shows a marked preference for prephenate as substrate compared to pyruvate, phenylpyruvate or 4-hydroxyphenylpyruvate.
In bacteria, the reaction is carried out (as a promiscuous activity) by enzymes with aspartate aminotransferase, branched-chain amino acid aminotransferase and N-succinyldiaminopymelate aminotransferase activities.
Organisms -Eubacteria -Plants

Family (9)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Giustini C, Graindorge M, Cobessi D, Crouzy S, Robin A, Curien G, Matringe M (2019) Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase FEBS J 286 2118-2134.

 Holland CK, Berkovich DA, Kohn ML, Maeda H, Jez JM (2018) Structural basis for substrate recognition and inhibition of prephenate aminotransferase from Arabidopsis Plant J 94 304-314.

 de la Torre F, El-Azaz J, Avila C, Cánovas FM (2014) Deciphering the role of aspartate and prephenate aminotransferase activities in plastid nitrogen metabolism Plant Physiol 164 92-104.

 Graindorge M, Giustini C, Kraut A, Moyet L, Curien G, Matringe (2014) Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms J Biol Chem 289 3198-208.

 Graindorge M, Giustini C, Jacomin AC, Kraut A, Curien G, Matringe M. (2010) Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: The last homeless enzyme of aromatic amino acids biosynthesis FEBS Lett 584 4357-60.

 Maeda H, Yoo H, Dudareva N. (2010) Prephenate aminotransferase directs plant phenylalanine biosynthesis via arogenate Nat Chem Biol 7 19-21.

 Bonner, C.; Jensen, R. (1985) Novel features of prephenate aminotransferase from cell cultures of Nicotiana silvestris Arch Biochem Biophys 238 237-46.

Articles on
last changed 2019/06/21 13:05

B6db activities