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B6db activities: 4.4.1.11

4.4.1.11
Description Methionine gamma-lyase
Alternative names L-methioninase;
Methionine lyase;
L-methionine-alpha-deamino-gamma-mercaptomethane-lyase;
METase.
Catalyzed reaction L-methionine = methanethiol + NH(3) + 2-oxobutanoate.
Cofactor Pyridoxal-phosphate.
Comments A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing methanethiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form 2-oxobutanoate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme is involved in L-methionine catabolism.
Prosite PROSITE; PDOC00677;
PDB 1GC0; 1E5F; 5DX5; 4U1T;
Organisms -Eubacteria -Plants -Fungi -Metazoa
 

Family 

4.4.1.11 (32)
 
Links Enzyme (activities) 4.4.1.11
BRENDA (activities) 4.4.1.11
KEGG (pathways) 4.4.1.11
PLPMDB (PLP mutants) 4.4.1.11
 
References
 El-Sayed AS, Ruff LE, Ghany SE, Ali GS, Esener S (2017) Molecular and Spectroscopic Characterization of Aspergillus flavipes and Pseudomonas putida L-Methionine γ-Lyase in Vitro Appl Biochem Biotechnol 181 1513-32.

 Kannan Suganya K, Govindan K, Prabha P, Murugan M (2017) An extensive review on L-methioninase and its potential applications Biocatalysis and Agricultural Biotechnology 12 104-15.

 Kulikova VV, Morozova EA, Revtovich SV, Kotlov MI, Anufrieva NV, Bazhulina NP, Raboni S, Faggiano S, Gabellieri E, Cioni P, Belyi YF, Mozzarelli A, Demidkina TV (2017) Gene cloning, characterization, and cytotoxic activity of methionine γ-lyase from Clostridium novyi IUBMB Life 69 668-676.

 Revtovich S, Anufrieva N, Morozova E, Kulikova V, Nikulin A, Demidkina T (2016) Structure of methionine γ-lyase from Clostridium sporogenes Acta Crystallograph Sect F Struct Biol Cryst Commun. 72 65-71.

 Huang KY, Hu HY, Tang YL, Xia FG, Luo XQ, Liu J (2015) High-Level Expression, Purification and Large-Scale Production of l-Methionine γ-Lyase from Idiomarina as a Novel Anti-Leukemic Drug Mar Drugs 13 5492-507.

 Kudou D, Yasuda E, Hirai Y, Tamura T, Inagaki K (2015) Molecular cloning and characterization of l-methionine γ-lyase from Streptomyces avermitilis J Biosci Bioeng 120 380-3.

 Song H, Xu R, Guo Z (2015) Identification and characterization of a methionine γ-lyase in the calicheamicin biosynthetic cluster of Micromonospora echinospora Chembiochem 16 100-9.

 Gonda I, Lev S, Bar E, Sikron N, Portnoy V, Davidovich-Rikanati R, Burger J, Schaffer AA, Tadmor Y, Giovannonni JJ, Huang M, Fei Z, Katzir N, Fait A, Lewinsohn E (2013) Catabolism of L-methionine in the formation of sulfur and other volatiles in melon (Cucumis melo L.) fruit Plant J 74 458-72.

 Morozova EA, Kulikova VV, Yashin DV, Anufrieva NV, Anisimova NY, Revtovich SV, Kotlov MI, Belyi YF, Pokrovsky VS, Demidkina TV (2013) Kinetic Parameters and Cytotoxic Activity of Recombinant Methionine γ-Lyase from Clostridium tetani, Clostridium sporogenes, Porphyromonas gingivalis and Citrobacter freundii Acta Naturae 5 92-8.

 Revtovich SV, Morozova EA, Anufrieva NV, Kotlov MI, Belyi YF, Demidkina TV (2012) Identification of methionine γ-lyase in genomes of some pathogenic bacteria Dokl Biochem Biophys 445 187-93.

 Suwabe K, Yoshida Y, Nagano K, Yoshimura F. (2011) Identification of an L-methionine γ-lyase involved in the production of hydrogen sulfide from L-cysteine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586 Microbiology 157 2992-3000.

 Goyer A, Collakova E, Shachar-Hill Y, Hanson AD (2007) Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway Plant Cell Physiol 48 232-42.

 Fukamachi, H.; Nakano, Y.; Okano, S.; Shibata, Y.; Abiko, Y.; Yamashita, Y. (2005) High production of methyl mercaptan by L-methionine-alpha-deamino-gamma-mercaptomethane lyase from Treponema denticola Biochem Biophys Res Commun 331 127-31.

 Mamaeva, D.V.; Morozovam, E.A.; Nikulinm, A.D.; Revtovichm, S.V.; Nikonovm, S.V.; Garberm, M.B.; Demidkina, T.V. (2005) Structure of Citrobacter freundiiL-methionine gamma-lyase Acta Crystallograph Sect F Struct Biol Cryst Commun. 61 546-9.

 Manukhov, IV, Mamaeva, DV, Rastorguev, SM, Faleev, NG, Morozova, EA, Demidkina, TV, Zavilgelsky, GB. (2005) A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase J Bacteriol 187 3889-93.

 Tokoro, M.; Asai, T.; Kobayashi, S.; Takeuchi, T.; Nozaki, T. (2003) Identification and characterization of two isoenzymes of methionine gamma-lyase from Entamoeba histolytica: a key enzyme of sulfur-amino acid degradation in an anaerobic parasitic protist that lacks forward and reverse trans-sulfuration pathways J Biol Chem 278 42717-27.

 Motoshima, H.; Inagaki, K.; Kumasaka, T.; Furuichi, M.; Inoue, H.; Tamura, T.; Esaki, N.; Soda, K.; Tanaka, N.; Yamamoto, M.; Tanaka, H. (2000) Crystal structure of the pyridoxal 5'-phosphate dependent L-methionine gamma-lyase from Pseudomonas putida J Biochem (Tokyo) 128 349-54.

 Yoshimura, M.; Nakano, Y.; Yamashita, Y.; Oho, T.; Saito, T.; Koga, T. (2000) Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis Infect Immun 68 6912-6.

 McKie, A. E.; Edlind, T.; Walker, J.; Mottram, J. C.; Coombs, G. H. (1998) The primitive protozoon Trichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes J Biol Chem 273 5549-56.

 Hori H, Takabayashi K, Orvis L, Carson DA, Nobori T. (1996) Gene cloning and characterization of Pseudomonas putida L-methionine-alpha-deamino-gamma-mercaptomethane-lyase Cancer Res 56 2116-22.

Articles on 4.4.1.11
 
last changed 2019/06/20 13:22

B6db activities