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B6db activities:
Description Hercynylcysteine S-oxide lyase
Alternative names hercynylcysteine sulfoxide lyase;
EgtE (gene name)
Catalyzed reaction S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor = ergothioneine + pyruvate + ammonia + acceptor (overall reaction)
(1a) S-(hercyn-2-yl)-L-cysteine S-oxide + H(2)O = 2-(hydroxysulfanyl)hercynine + pyruvate + NH(3).
(1b) 2-(hydroxysulfanyl)hercynine + reduced acceptor = ergothioneine + acceptor + H(2)O (spontaneous)
Cofactor Pyridoxal phosphate.
Comments The enzyme, characterized from the bacterium Mycobacterium smegmatis, cayalyses the last step in the pathway of ergothioneine biosynthesis. The enzyme forms a 2-(hydroxysulfanyl)hercynine intermediate, which is reduced to ergothioneine non-enzymically by a thiol. In vitro, DTT can serve this function.
Ergothioneine is a a thiourea derivative of histidine. This small, sulfur-containing metabolite (229 Da) is synthesized by various species of bacteria and fungi, and can accumulate to millimolar levels in tissues or cells (e.g. erythrocytes) of higher eukaryotes.
Organisms -Eubacteria -Fungi

Family (0)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
References Articles on
last changed 2017/12/21 12:49

B6db activities