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B6db activities: chdn

Description 9-desmethyl-chelocardin aminotransferase ( 2.6.1.-)
Alternative names ChdN (gene name)
Catalyzed reaction 9-desmethyl-4-oxo-chelocardin + an L-amino acid = 9-desmethyl-chelocardin + a 2-oxo acid
Cofactor Pyridoxal-phosphate.
In the biosynthesis of chelocardin antibiotics (very similar to tetracyclines) the aminotransferase ChdN catalyzes the amination of C4-keto of 9-desmethyl-4-oxo-chelocardin, yielding 9-desmethyl-chelocardin. The amino carbon stereochemistry is (S), opposite to that of the enzymes in the OxyQ family, that act on a similar substrate.

The amino group donor is not specified, because a soluble version of the recombinant protein has not yet been studied.

Organisms -Eubacteria


chdn (0)
Links Enzyme (activities) chdn
BRENDA (activities) chdn
KEGG (pathways) chdn
PLPMDB (PLP mutants) chdn
 Lukežič; T, Fayad AA, Bader C, Harmrolfs K, Bartuli J, Groß S, Lešnik U, Hennessen F, Herrmann J, Pikl Š, Petković H, Müller R (2019) Engineering Atypical Tetracycline Formation in Amycolatopsis sulphurea for the Production of Modified Chelocardin Antibiotics ACS Chem Biol 14 468-477.

 Lukežič T, Lešnik U, Podgoršek A, Horvat J, Polak T, Šala M, Jenko B, Raspor P, Herron PR, Hunter IS, Petković H (2013) Identification of the chelocardin biosynthetic gene cluster from Amycolatopsis sulphurea: a platform for producing novel tetracycline antibiotics Microbiology 29 2524-32.

Articles on chdn
last changed 2019/07/29 12:14

B6db activities