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B6db activities: cqsa

cqsa
Description Acyl-CoA:L-2-aminobutyrate C-acyltransferase (decarboxylating) (2.3.1.-)
Alternative names Cqsa;
Lqsa;
CAI-1 autoinducer synthase;
LAI-1 autoinducer synthase.
Catalyzed reaction Acyl-CoA+ L-2-aminobutanoate = CoA + 3-amino-4-alkanone + CO(2)
Cofactor Pyridoxal phosphate
Comments In Vibrio and Legionella species these enzymes are involved in the biosynthesis of 3-hydroxy-4-keto autoinducer molecules (such as 3-hydroxytridecan-4-one, better known as CAI-1), responsible for quorum sensing.

The precise reaction catalyzed by these enzymes is not known. The Vibrio enzyme can use as substrates decanyl-CoA and 2-aminobutyrate, giving the reaction above, but the physiological amino acid substrate could be different. Furthermore, there are indirect evidences that acyl-CoAs of different length can be used by these enzymes.

PDB 2WKA; 2WK8;
Organisms -Eubacteria
 

Family 

cqsa (12)
 
Links Enzyme (activities) cqsa
BRENDA (activities) cqsa
KEGG (pathways) cqsa
PLPMDB (PLP mutants) cqsa
 
References
 Jahan N, Potter JA, Sheikh MA, Botting CH, Shirran SL, Westwood NJ, Taylor GL (2009) Insights into the Biosynthesis of the Vibrio cholerae Major Autoinducer CAI-1 from the Crystal Structure of the PLP-Dependent Enzyme CqsA J Mol Biol 393 763-73.

 Kelly RC, Bolitho ME, Higgins DA, Lu W, Ng WL, Jeffrey PD, Rabinowitz JD, Semmelhack MF, Hughson FM, Bassler BL. (2009) The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA Nat Chem Biol 5 891-5.

 Spirig T, Tiaden A, Kiefer P, Buchrieser C, Vorholt JA, Hilbi H. (2008) The Legionella autoinducer synthase LqsA produces an alpha-hydroxyketone signaling molecule J Biol Chem 283 18113-23.

Articles on cqsa
 
last changed 2009/10/21 16:05

B6db activities