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kdna |
| Description |
kdna-like transaminase (2.6.1.-) |
| Catalyzed reaction |
3-deoxy-8oxy-D-manno-octulosonic acid + L-glutamate = 3-deoxy-8-deoxy-8-amino-D-manno-octulosonic acid + α-ketoglutarate |
| Cofactor |
Pyridoxal-phosphate. |
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Lipopolysaccharide (LPS) is composed of a hydrophobic anchor, known as lipid A, an inner core oligosaccharide, and a repeating O-antigen polysaccharide. In nearly all species, the first sugar bridging the hydrophobic lipid A and the polysaccharide domain is 3-deoxy-d-manno-octulosonic acid (Kdo), and thus it is critically important for LPS biosynthesis. Modifications to lipid A have been shown to be important for resistance to antimicrobial peptides as well as modulating recognition by the mammalian innate immune system. Therefore, lipid A derivatives have been used for development of vaccine strains and vaccine adjuvants. One derivative that has yet to be studied is 8-amino-3,8-dideoxy-d-manno-octulosonic acid (Kdo8N), which is found exclusively in marine bacteria of the genus Shewanella. Expression of these genes recombinantly in Escherichia coli resulted in lipid A containing Kdo8N, and in vitro assays confirmed their proposed enzymatic function. |
| Organisms |
-Eubacteria |
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Family |
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| Links |
Enzyme (activities) kdna
BRENDA (activities) kdna
KEGG (pathways) kdna
PLPMDB (PLP mutants) kdna
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| References |
Articles on kdna |
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| last changed |
2014/03/03 14:04 |
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