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B6db activities: mcce

Description Microcin biosynthesis intermediate decarboxylase (4.1.1.-)
Alternative names Microcin c synthase
Catalyzed reaction Microcin intermediate (1120)=>Mature microcin C(1177)+CO(2)
Cofactor Pyridoxal-phosphate.
The enzyme from E. coli is involved in the Microcin C (McC) maturation pathway.
This Trojan horse antibiotic McC consists of a heptapeptide attached to adenosine through a phosphoramidate linkage. α -carboxyl group linked to AMP via phosphoramidate bond.

Two enzymes, MccE and MccD, act in tandem to add to the McC phosphate an aminopropyl moiety (this increases the biological activity by ∼ 10-fold).
Specifically, MccD transfers a 3-amino-3-carboxypropyl group S-adenosyl methionine onto the phosphate of the adenylated heptapeptide. Subsequently, MccE removes the carboxyl group, yielding mature McC.

Organisms -Eubacteria


mcce (0)
Links Enzyme (activities) mcce
BRENDA (activities) mcce
KEGG (pathways) mcce
PLPMDB (PLP mutants) mcce
 Bantysh O, Serebryakova M, Makarova KS, Dubiley S, Datsenko KA, Severinov K (2014) Enzymatic synthesis of bioinformatically predicted microcin C-like compounds encoded by diverse bacteria MBio 5 e01059-14.

 Kulikovsky A, Serebryakova M, Bantysh O, Metlitskaya A, Borukhov S, Severinov K, Dubiley S. (2014) The molecular mechanism of aminopropylation of peptide-nucleotide antibiotic microcin C J Am Chem Soc 136 11168-75.

Articles on mcce
last changed 2019/06/20 13:09

B6db activities