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B6db activities: r-amine.pyruvate.aminotransferase

r-amine:pyruvate_aminotransferase
Description (R)-amine:pyruvate aminotransferase (2.6.1.-)
Catalyzed reaction (R)-2-amine + pyruvate = 2- oxo-compound + D-alanine
Cofactor Pyridoxal-phosphate.
A novel (R)-amine transaminase, which catalyzed (R)-enantioselective transamination of chiral amine, was purified to homogeneity from Arthrobacter sp. KNK168. The enzyme catalyzed transamination between amines and pyruvate stereo-specifically. The deduced amino acid sequence was found to be homologous to those of the aminotransferases belonging to fold class IV of pyridoxal-5'-phosphate-dependent enzymes, such as branched-chain amino acid aminotransferases.
Organisms -Eubacteria
 

Family 

 
Links Enzyme (activities) r-amine:pyruvate_aminotransferase
BRENDA (activities) r-amine:pyruvate_aminotransferase
KEGG (pathways) r-amine:pyruvate_aminotransferase
PLPMDB (PLP mutants) r-amine:pyruvate_aminotransferase
 
References
 Łyskowski A, Gruber C, Steinkellner G, Schürmann M, Schwab H, Gruber K, Steiner K. (2014) Crystal structure of an (R)-selective ω-transaminase from Aspergillus terreus. PLoS One. 9 .

 Iwasaki A, Matsumoto K, Hasegawa J, Yasohara Y. (2012) A novel transaminase, (R)-amine:pyruvate aminotransferase, from Arthrobacter sp. KNK168 (FERM BP-5228): purification, characterization, and gene cloning. Appl Microbiol Biotechnol. 93 1563-73.

Articles on r-amine.pyruvate.aminotransferase
 
last changed 2014/04/16 13:32

B6db activities