Activities | Families | Sequences | Fold types | References | Help
B6db activities: vioa

vioa
Description dTDP-4-dehydro-6-deoxy-D-glucose aminotransferase (2.6.1.-)
Alternative names dTDP-4-keto-6-deoxy-D-glucose-oxoglutarate aminotransferase;
dTDP-4-amino-4,6-dideoxy-D-glucose--glutamate aminotransferase;
D-viosamine synthase;
VioA;
GerB;
DesI.
Catalyzed reaction dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate.
Cofactor Pyridoxal phosphate.
Comments The enterobacterial enzyme is responsible for the synthesis of D-Viosamine (4-amino-4,6-dideoxy-D-glucose), whose derivatives, such as 4-(N-acetylglycyl)amino-4,6-dideoxy-D-glucose, are component of O-antigen variants.
In Streptomyces, the same reaction is exploited in metabolic pathways leading to the production of macrolides.
PDB 2PO3;
Organisms -Eubacteria
 

Family 

vioa (21)
 
Links Enzyme (activities) vioa
BRENDA (activities) vioa
KEGG (pathways) vioa
PLPMDB (PLP mutants) vioa
 
References
 Dong S, McPherson SA, Wang Y, Li M, Wang P, Turnbough CL Jr, Pritchard DG. (2010) Characterization of the enzymes encoded by the anthrose biosynthetic operon of Bacillus anthracis J Bacteriol 192 5053-62.

 Burgie ES, Holden HM. (2007) Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine Biochemistry 46 8999-9006.

 Chung YS, Kim DH, Seo WM, Lee HC, Liou K, Oh TJ, Sohng JK. (2007) Enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-D-glucose using GerB (dTDP-4-keto-6-deoxy-D-glucose aminotransferase) Carbohydr Res 342 1412-8.

 Wang Y, Xu Y, Perepelov AV, Qi Y, Knirel YA, Wang L, Feng L. (2007) Biochemical characterization of dTDP-D-Qui4N and dTDP-D-Qui4NAc biosynthetic pathways in Shigella dysenteriae type 7 and Escherichia coli O7 J Bacteriol 189 8626-35.

Articles on vioa
 
last changed 2009/07/29 13:24

B6db activities