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B6db references: 0..ceaagaa

type Journal Article
authors Nishijima, S.; Sugaya, K.; Morozumi, M.; Hatano, T.; Ogawa, Y.
title Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver
journal J Chromatogr B Analyt Technol Biomed Life Sci
ui 0; Ceaagaa
year (2002)
volume 780
number 1
pages 13.
abstract We measured hepatic alanine:glyoxylate aminotransferase (AGT) activity using capillary electrophoresis. After rat liver homogenate was incubated in the presence of substrates and pyridoxal 5'-phosphate, the pyruvate and glycine produced by AGT were measured. The AGT activity was 10.02+/-0.31 &mgr;mol pyruvate/h/mg protein and 10.21+/-0.15 &mgr;mol glycine/h/mg protein. This method is relatively simple and shows superior sensitivity, allowing the measurement of enzyme activity in 5 &mgr;g of protein. Therefore, it appears to be suitable for laboratory use and may also have advantages for measuring AGT activity in liver biopsy specimens.
last changed 2002/11/12 16:17

B6db references