|
type |
Journal Article |
authors |
Nishijima, S.; Sugaya, K.; Morozumi, M.; Hatano, T.; Ogawa, Y. |
title |
Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver |
journal |
J Chromatogr B Analyt Technol Biomed Life Sci |
Activity |
2.6.1.44 |
ui |
0; Ceaagaa |
year |
(2002) |
volume |
780 |
number |
1 |
pages |
13. |
| |
abstract |
We measured hepatic alanine:glyoxylate aminotransferase (AGT) activity using capillary electrophoresis. After rat liver homogenate was incubated in the presence of substrates and pyridoxal 5'-phosphate, the pyruvate and glycine produced by AGT were measured. The AGT activity was 10.02+/-0.31 &mgr;mol pyruvate/h/mg protein and 10.21+/-0.15 &mgr;mol glycine/h/mg protein. This method is relatively simple and shows superior sensitivity, allowing the measurement of enzyme activity in 5 &mgr;g of protein. Therefore, it appears to be suitable for laboratory use and may also have advantages for measuring AGT activity in liver biopsy specimens. |
last changed |
2002/11/12 16:17 |
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