|
type |
Journal Article |
authors |
Sienko, M.; Paszewski, A. |
title |
The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase: cloning and analysis |
journal |
Curr Genet |
Activity |
4.4.1.8 |
Family |
4.4.1.8.c |
sel |
selected |
ui |
10467009 |
year |
(1999) |
volume |
35 |
number |
6 |
pages |
638-46 |
| |
keywords |
Amino Acid Sequence |
abstract |
The metG gene of Aspergillus nidulans encoding cystathionine beta-lyase, an enzyme of the main pathway of methionine synthesis, was cloned by complementation of a metG mutation. A comparison of metG genomic DNA and a cDNA copy derived from different A. nidulans strains revealed a marked DNA sequence polymorphism manifested mostly by silent point mutations. cDNA of the A. nidulans metG gene complemented the Escherichia coli metC69 mutation impairing cystathionine beta-lyase. This gene contains two introns and codes for a protein of 439 amino acids. The protein shows homology with bacterial, yeast and plant cystathionine beta-lyases, as well as with other enzymes belonging to a large family of pyridoxal 5'-phosphate binding proteins. Transcription of the metG gene is not appreciably regulated by the concentration of sulphur source in the growth medium. |
last changed |
2009/01/08 14:14 |
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