|
type |
Journal Article |
authors |
Long, Z.; Lee, J. A.; Okamoto, T.; Sekine, M.; Nimura, N.; Imai, K.; Yohda, M.; Maruyama, T.; Sumi, M.; Kamo, N.; Yamagishi, A.; Oshima, T.; Homma, H. |
title |
Occurrence of D-Amino Acids and a pyridoxal 5'-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum |
journal |
Biochem Biophys Res Commun |
Activity |
5.1.1.13 |
sel |
selected |
ui |
11181048 |
year |
(2001) |
volume |
281 |
number |
2 |
pages |
317-21 |
| |
keywords |
Alanine/chemistry/metabolism |
abstract |
Free D-amino acid content in some archaea was investigated and D-forms of several amino acids were found in them. In the acidothermophilic archaeon, Thermoplasma acidophilum, the proportion of D-aspartate (D- Asp) to total Asp was as high as 39.7%. Crude extracts of Thermoplasma acidophilum had Asp-specific racemase activity that was pyridoxal 5'- phosphate (PLP)-dependent. The relative insensitivity to a SH-modifying reagent distinguished this activity from those of the PLP-independent Asp racemases found in other hyperthermophilic archaea (Matsumoto, M., et al., J. Bacteriol. 181, 6560-6563 1999). Thus, high levels of d-Asp should be produced by a new type(s) of Asp-specific racemase in Thermoplasma acidophilum, although the function of d-Asp in this archaeon remains unknown. |
last changed |
2009/07/02 14:26 |
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