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B6db references: 11191214

type Journal Article
authors Elrod, S.L.; Jones, A.; Berka, R.M.; Cherry, J.R.
title Cloning of the Aspergillus oryzae 5-aminolevulinate synthase gene and its use as a selectable marker
journal Curr Genet
sel selected
ui 11191214
year (2000)
volume 38
number 5
pages 291-98
keywords 5-Aminolevulinate Synthetase/genetics/*metabolism
abstract The hemA gene encoding 5-aminolevulinate synthase, the first enzyme in heme biosynthesis, was cloned from Aspergillus oryzae and evaluated as a selectable marker for the transformation of filamentous fungi. Deletion of the hemA gene resulted in a lethal phenotype that could be rescued either by the supplementation of culture media with 5-aminolevulinic acid (ALA) or by transformation with the wild-type hemA gene, but not by growth on rich media, nor by the addition of exogenous heme. Transformation of a hemA deletion strain with the hemA gene linked to a lipase expression cassette yielded ALA prototrophs expressing lipase. The hemA gene can therefore be used as a selectable marker for the transformation of A. oryzae.
last changed 2009/01/12 19:14

B6db references