Activities | Families | Sequences | Fold types | References | Help
B6db references: 11500565

type Journal Article
authors Lopukhina, A.; Dettenberg, M.; Weiler, E.W.; Hollander-Czytko, H.
title Cloning and characterization of a coronatine-regulated tyrosine aminotransferase from Arabidopsis
journal Plant Physiol
sel selected
ui 11500565
year (2001)
volume 126
number 4
pages 1678-87
abstract In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronatine-treated Arabidopsis. Among these, a cDNA clone was identified that was similar to known tyrosine aminotransferases (TATs). The function was verified with the expressed recombinant protein. In Arabidopsis, the protein is present as a multimer of 98 kD, with a monomer of an apparent molecular mass of 47 kD. TAT mRNA could be induced within 2 h by various octadecanoids and by wounding of the plants. Accumulation of the TAT protein and a 5- to 7-fold increase in its enzymatic activity was observed 7 to 9 h after application of octadecanoids, coronatine, or wounding. The potential role of TAT in the defense response to herbivores and pathogens is discussed.
last changed 2019/05/13 09:56

B6db references