|
type |
Journal Article |
authors |
Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K. |
title |
Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans |
journal |
J Antibiot (Tokyo) |
Activity |
2.6.1.100 |
Family |
2.6.1.100 |
sel |
selected |
ui |
12374384 |
year |
(2002) |
volume |
55 |
number |
8 |
pages |
707-714 |
| |
abstract |
Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.
|
last changed |
2017/11/03 17:59 |
|