|
type |
Journal Article |
authors |
Herr, D.R.; Fyrst, H.; Phan, V.; Heinecke, K.; Georges, R.; Harris, G.L.; Saba, J.D.
|
title |
Sply regulation of sphingolipid signaling molecules is essential for Drosophila development |
journal |
Development |
Activity |
4.1.2.27 |
sel |
selected |
ui |
12702658 |
year |
(2003) |
volume |
130 |
number |
18 |
pages |
2443-53 |
| |
abstract |
Sphingosine-1-phosphate is a sphingolipid metabolite that regulates cell proliferation, migration and apoptosis through specific signaling pathways. Sphingosine-1-phosphate lyase catalyzes the conversion of sphingosine-1-phosphate to ethanolamine phosphate and a fatty aldehyde. We report the cloning of the Drosophila sphingosine-1-phosphate lyase gene (Sply) and demonstrate its importance for adult muscle development and integrity, reproduction and larval viability. Sply expression is temporally regulated, with onset of expression during mid-embryogenesis. Sply null mutants accumulate both phosphorylated and unphosphorylated sphingoid bases and exhibit semi-lethality, increased apoptosis in developing embryos, diminished egg-laying, and gross pattern abnormalities in dorsal longitudinal flight muscles. These defects are corrected by restoring Sply expression or by introduction of a suppressor mutation that diminishes sphingolipid synthesis and accumulation of sphingolipid intermediates. This is the first demonstration of novel and complex developmental pathologies directly linked to a disruption of sphingolipid catabolism in metazoans. |
last changed |
2009/06/23 09:13 |
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