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B6db references: 1418690

type Journal Article
authors Iwamori, S.; Oikawa, T.; Ishiwata, K.; Makiguchi, N.
title Cloning and expression of the Erwinia herbicola tyrosine phenol-lyase gene in Escherichia coli
journal Biotechnol Appl Biochem
sel selected
ui 1418690
year (1992)
volume 16
number 1
pages 77-85
keywords Amino Acid Sequence
abstract The tyrosine phenol-lyase (TPL) gene of Erwinia herbicola was cloned and expressed in Escherichia coli, and the complete nucleotide sequence of the gene determined. The TPL gene comprises 1368 bp, encoding 456 amino acids which have 90% amino acid identity with TPL from Citrobacter freundii. After replacing the 5'-flanking region of the TPL gene with the E. coli lac promoter, TPL protein could be hyperproduced constitutively in E. coli without induction by L-tyrosine.
last changed 2009/06/23 09:45

B6db references