|
type |
Journal Article |
authors |
Sun, L.; Li X, Dong, Y.; Yang, M.; Liu, Y.; Han, X.; Zhang, X.; Pang, H.; Rao, Z. |
title |
Crystallization and preliminary crystallographic analysis of human serine dehydratase |
journal |
Acta Crystallogr D Biol Crystallogr |
Activity |
4.3.1.17 |
sel |
unselected |
ui |
14646100 |
year |
(2003) |
volume |
59 |
number |
12 |
pages |
2297-9. |
| |
abstract |
L-Serine dehydratase (SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of L-serine to yield pyruvate. Recombinant human serine dehydratase was crystallized by the hanging-drop vapour-diffusion method. Crystals were grown at 291 K using (NH4)(2)SO4 as precipitant. Diffraction data were obtained to a resolution of 2.5 A from a single frozen crystal using Cu Kalpha radiation. The crystal belongs to space group I422, with unit-cell parameters a = 157.4, b = 157.4, c = 59.2 A, alpha = beta = gamma = 90 degrees. The asymmetric unit contains one molecule and has a solvent content of about 46%. |
last changed |
2008/03/27 19:30 |
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