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B6db references: 15582417

type Journal Article
authors Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K.
title Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius
journal Bioorg Med Chem Lett
Activity 2.6.1.50
Family 2.6.1.50
sel selected
ui 15582417
year (2005)
volume 15
pages 89-92
 
abstract 2-Deoxystreptamine (DOS)-containing aminoglycoside–aminocyclitol (AmAc) antibiotics represent the majority of clinically important AmAcs. Biosynthetic investigations of formation of DOS in actinomycetes are limited to the characterization of 2-deoxy-scyllo-inosose synthase, the first step enzyme of the DOS biosynthetic pathway. A gene encoding L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from the tobramycin producer Streptomyces tenebrarius was expressed heterologously in Escherichia coli. The conversions of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and scyllo-inosose to scyllo-inosamine with the activity of TbmB were determined in vitro. The results indicate that tbmB catalyzes the second step of the DOS biosynthetic pathway during the biosynthesis of 2-deoxystreptamine, a subunit of tobramycin, in S. tenebrarius.
last changed 2009/01/08 15:06

B6db references